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An Efficient and Flexible Cell Aggregation Method for 3D Spheroid Production

DOI :

10.3791/55544-v

March 27th, 2017

March 27th, 2017

22,729 Views

1Division of Cancer Biology and Genetics, Queen's University Cancer Research Institute, 2Department of Pathology and Molecular Medicine, Queen's University

Here, we describe a rapid and flexible protocol for the formation of 3D cell spheroids through cell aggregation. This is easily adapted to multiple cell types and is suitable for use in a variety of applications including cell migration, invasion, or anoikis assays, and for imaging and quantifying cell-matrix interactions.

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Keywords 3D Spheroid Production

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