July 24th, 2017
•This protocol describes real-time iontophoresis, a method that measures physical parameters of the extracellular space (ECS) of living brains. The diffusion of an inert molecule released into the ECS is used to calculate the ECS volume fraction and tortuosity. It is ideal for studying acute reversible changes to brain ECS.
Related Videos
Fast Micro-iontophoresis of Glutamate and GABA: A Useful Tool to Investigate Synaptic Integration
Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons
An Ex Vivo Laser-induced Spinal Cord Injury Model to Assess Mechanisms of Axonal Degeneration in Real-time
Real-time Electrophysiology: Using Closed-loop Protocols to Probe Neuronal Dynamics and Beyond
A Protocol for the Administration of Real-Time fMRI Neurofeedback Training
Assay to Measure Nucleocytoplasmic Transport in Real Time within Motor Neuron-like NSC-34 Cells
Live-cell Measurement of Odorant Receptor Activation Using a Real-time cAMP Assay
Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System
Exploring Deep Space - Uncovering the Anatomy of Periventricular Structures to Reveal the Lateral Ventricles of the Human Brain
In Vivo Microdialysis Method to Collect Large Extracellular Proteins from Brain Interstitial Fluid with High-molecular Weight Cut-off Probes
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved