Name: real-time in vitro monitoring of odorant receptor activation by an odorant in the vapor phase
Uploaded: 2019-04-23T14:00:00
Description: Watch this Scientific Journal Video about Real-time In Vitro Monitoring of Odorant Receptor Activation by an Odorant in the Vapor Phase at JoVE.com

This work was supported by grants from NIH (DC014423 and DC016224) and the Defense Advanced Research Project Agency RealNose Project.&#160;YF stayed at Duke University with financial support from JSPS Program for Advancing Strategic International Networks to Accelerate the Circulation of Talented Researchers (R2801). We thank Sahar Kaleem for editing of the manuscript.

1. Hana3A Cells Culture
<ol>
	<li>Prepare M10 (Minimum Essential Medium (MEM) plus 10 % v/v fetal bovine serum (FBS)) and M10PSF (M10 plus 100 &#181;g/mL penicillin-streptomycin and 1.25 &#181;g/mL amphotericin B).</li>
	<li>Culture the cells in 10 mL of M10PSF in a 100 mm cell culture dish in an incubator set at 37 &#176;C and 5% carbon dioxide (CO2).</li>
	<li>Divide the cells every 2 days at a 20% ratio: when 100% confluence of cells (approximately 1.1 x 107 cells) is observed under a phase-con...

The perception of odor is fundamentally dependent on the activation of ORs. Consequently, understanding of their functionality is required to crack the complex mechanisms that the brain use to perceive its volatile chemical environment. However, the understanding of this process has been hampered by the difficulties in establishing a robust method to screen the OR repertoire for functionality against odorants in vitro. Cell surface and heterologous expression of ORs has been partially solved by the creation of t...

The sense of smell allows terrestrial animals to interact with their volatile chemical environment to drive behaviors and emotions. Fundamentally, the odor detection process begins with the very first interaction of odorant molecules with the olfactory system, at the level of odorant receptors (ORs)1. In mammals, ORs are individually expressed in olfactory sensory neurons (OSNs) located in the olfactory epithelium2. They belong to the G-protein coupled receptor (GPCR) family and more precisely to the rhodopsin-like sub-family (also called class A). ORs couple with the stimulatory G protein Golf whose activation leads to cAMP production followed by the opening of cyclic nucleotide gated channels and the generation of action potentials. It is accepted that an odor percept relies on a specific pattern of activated ORs3,4 and therefore odor recognition follows a combinatorial coding scheme, where one OR can be activated by a set of odorants and one odorant can activate a combination of ORs. And through such combinatorial coding, it is postulated that organisms can detect and discriminate between a myriad of volatile odor molecules. One of the keys to understanding how odors are perceived is to understand how and which ORs are activated by a given odor.
In an attempt to elucidate odorant-OR interactions, in vitro functional assays have played an essential role. The identification of agonist odorous ligands for orphan ORs (OR de-orphanization) has been a very active field for the past twenty years, through the use of various in vitro, ex vivo and in vivo functional assays5,6,7,8,9,10,11,12,13,14,15,16,17.
In vitro assay systems are best suited for the detailed functional characterization of ORs, including identifying the functional domains and critical residues of ORs, as well as potential engineering applications. However, further development of valuable in vitro systems for ORs has been a challenge, in part due to difficulty with culturing OSNs and functional expression of ORs in heterologous cells. The first challenge had been to establish protocols that allowed for the cell surface expression of functional ORs in the mapping of odorant-OR interactions. A number of independent groups have utilized various approaches5,6,7,8,9,10,11,12,14,18,19,20. One of the earliest achievements was made by Krautwurst et al. in tagged the N-terminus of ORs with a shortened sequence of rhodopsin (Rho-tag) and observed an improved surface expression in human embryonic kidney (HEK) cells13. Variations made to the tag attached to the OR sequence is still a path explored for improving OR expression and functionality19,21. Saito et al. then identified receptor-transporting protein 1 (RTP1) and RTP2 which facilitate OR trafficking.22 A shorter version of RTP1, called RTP1S, has also been shown to be even more effective than the original protein23. The development of a cell line (Hana3A) which stably expresses Golf, REEP1, RTP1, and RTP2 24, coupled with the use of cyclic adenosine monophosphate (cAMP) reporters has enabled identification of odorant-OR interactions. The mechanism by which the RTP family of proteins promotes cell surface expression of ORs remains to be determined.
One caveat of these established methods is that they rely on odorant stimulation in liquid phase, meaning that odorants are pre-dissolved into a stimulation medium and stimulate cells by replacing the medium. This is very distinct from the physiological conditions where odorant molecules reach the olfactory epithelium in vapor phase and activate ORs by dissolution into the nasal mucosa. To more closely resemble physiologically relevant stimulus exposure, Sanz et al.20 proposed an assay based on vapor stimulation by applying a drop of odorant solution to hang beneath the inner face of a plastic film placed on the top of cell wells. They recorded the calcium responses by monitoring fluorescence intensity. This method was the first to use air-phase odorant stimulation, but it did not allow a large screening of OR activation.
Here, we developed a new method that enables real-time monitoring of in vitro OR activation via vapor phase odorant stimulation by the Glosensor assay (Figure 1). This assay has been used previously in the context of liquid odorant stimulation18,19,25,26,27,28,29,30,31. The monitoring chamber of the luminometer is first equilibrated with vaporized odorant prior to plate reading (Figure 1A). Odorant molecules are then solvated into the buffer, bathing Hana3A cells expressing the OR of interest, RTP1S and the Glosensor proteins (Figure 1B). If the odorant is an agonist of the OR, the OR will switch to an activated conformation and bind the Golf, activating the adenylyl cyclase (AC), and ultimately cause cAMP levels to rise. This rising cAMP will bind to and activate the Glosensor protein to generate luminescence catalyzing luciferin. This luminescence is then recorded by the luminometer and enables OR activation monitoring. This method is of high interest in the context of OR deorphanization as it brings in vitro systems closer to the natural perception of odors.

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			<td height="22" style="height:22px;width:205px;">0.05 % trypsin-EDTA</td>
			<td style="width:93px;">Gibco</td>
			<td style="width:119px;">25300-054</td>
			<td style="width:449px;">0.05% Trypsin - EDTA (1x), phenol red - store at 4&#176;C</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">100 mm cell culture dish&#160;</td>
			<td>BD Falcon</td>
			<td>353003</td>
			<td>100 mm x 20 mm cell culture dish&#160;</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">15 mL tube</td>
			<td>BD Falcon</td>
			<td>352099</td>
			<td style="width:449px;">17 mm x 120 mm conical tubes</td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:205px;">96-well plate</td>
			<td style="width:93px;">Corning</td>
			<td style="width:119px;">3843</td>
			<td style="width:449px;">96 well, with LE lid white with clear bottom Poly-D-lysine coated Polystyrene</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:205px;">Amphotericin</td>
			<td style="width:93px;">Gibco</td>
			<td style="width:119px;">15290-018</td>
			<td style="width:449px;">Amphotericin B 250 &#181;g/mL - store at 4&#176;C</td>
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		<tr height="21">
			<td height="21" style="height:21px;">centrifuge machine</td>
			<td>Jouan</td>
			<td>C312</td>
			<td style="width:449px;">Centrifuge machine with swinging bucket rotor for 15 mL</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Class II Type A/B3 fumehood</td>
			<td>NUAIRE</td>
			<td>NU-407-500</td>
			<td style="width:449px;">fumehood for cell culturing</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:205px;">FBS</td>
			<td style="width:93px;">Gibco</td>
			<td style="width:119px;">16000-044</td>
			<td style="width:449px;">Fetal Bovine Serum - store at -20&#176;C</td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:205px;">GloSensor cAMP Reagent</td>
			<td style="width:93px;">Promega</td>
			<td style="width:119px;">E1290</td>
			<td style="width:449px;">GloSensor cAMP Reagent luminescent protein substrate - store at -20&#176;C</td>
		</tr>
		<tr height="24">
			<td height="24" style="height:24px;">Incubator 37 &#176;C; 5 % CO2</td>
			<td>Fisher Scientific</td>
			<td>11-676-604</td>
			<td style="width:449px;">Incubator for cell culturing</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">Lipofectamine 2000 reagent</td>
			<td>Invitrogen</td>
			<td>11668-019</td>
			<td style="width:449px;">Lipofectamine 2000 Reagent 1mg/ml transfection reagent - store at 4&#176;C</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Luminometer POLARstar OPTIMA</td>
			<td>BMG LABTECH</td>
			<td>discontinued</td>
			<td style="width:449px;">96 well plate reader for luminescence</td>
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		<tr height="20">
			<td height="20" style="height:20px;width:205px;">Mineral oil</td>
			<td style="width:93px;">Sigma</td>
			<td style="width:119px;">M8410</td>
			<td style="width:449px;">Solvent for odorants - store at room temperature</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:205px;">Minimum Essential Medium (MEM)</td>
			<td style="width:93px;">Corning cellgro</td>
			<td style="width:119px;">10-010-CV</td>
			<td style="width:449px;">Minimum Essential Medium Eagle with Earle&#8217;s salts &#38; L-glutamine - store at 4&#176;C</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:205px;">Penicillin/Streptomycin</td>
			<td style="width:93px;">Sigma Aldrich</td>
			<td style="width:119px;">P4333</td>
			<td style="width:449px;">Penicillin-Streptomycin solution stabilized with 10,000 U of penicillin and 10 mg streptomycin - store at -20&#176;C</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:205px;">pGlosensor</td>
			<td style="width:93px;">Promega</td>
			<td style="width:119px;">E2301</td>
			<td style="width:449px;">pGloSensor-22F cAMP luminescent protein plasmid - store at 4&#176;C</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">phase contrast microscope</td>
			<td>Leica</td>
			<td>090-131.001</td>
			<td style="width:449px;">phase contrast microscope with x4, x10, x20 objectives</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:205px;">RTP1S</td>
			<td style="width:93px;">H. Matsunami lab</td>
			<td style="width:119px;">-</td>
			<td style="width:449px;">100 ng/&#181;L plasmid - store at 4&#176;C</td>
		</tr>
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real-time in vitro monitoring of odorant receptor activation by an odorant in the vapor phase

Olfactory perception begins with the interaction of odorants with odorant receptors (OR) expressed by olfactory sensory neurons (OSN). Odor recognition follows a combinatorial coding scheme, where one OR can be activated by a set of odorants and one odorant can activate a combination of ORs. Through such combinatorial coding, organisms can detect and discriminate between a myriad of volatile odor molecules. Thus, an odor at a given concentration can be described by an activation pattern of ORs, which is specific to each odor. In that sense, cracking the mechanisms that the brain uses to perceive odor requires the understanding odorant-OR interactions. This is why the olfaction community is committed to &#34;de-orphanize&#34;&#160;these receptors. Conventional in vitro systems used to identify odorant-OR interactions have utilized incubating cell media with odorant, which is distinct from the natural detection of odors via vapor odorants dissolution into nasal mucosa before interacting with ORs. Here, we describe a new method that allows for real-time monitoring of OR activation via vapor-phase odorants. Our method relies on measuring cAMP release by luminescence using the Glosensor assay. It bridges current gaps between in vivo and in vitro approaches and provides a basis for a biomimetic volatile chemical sensor.

We screened the response of three mouse ORs, Olfr746, Olfr124 and Olfr1093 using cinnamaldehyde vapor stimulation (Figure 3). Simultaneously, we used an empty vector control (Rho-pCI) to assure that the odorant-induced activities of the tested ORs were specific (Figure 3A). The real-time activation of the ORs upon vapor odorant stimulus was monitored over 20 measurement cycles. The data for each well were first normalized to the empty vector con...

Watch this Scientific Journal Video about Real-time In Vitro Monitoring of Odorant Receptor Activation by an Odorant in the Vapor Phase at JoVE.com

Real-time In Vitro Monitoring of Odorant Receptor Activation by an Odorant in the Vapor Phase

Physiologically, odorant receptors are activated by odorant molecules inhaled in the vapor phase. However, most in vitro systems utilize liquid phase odorant stimulation. Here, we present a method that allows real-time in vitro monitoring of odorant receptor activation upon odorant stimulation in vapor phase.

Olfactory perception begins with the interaction of odorants with odorant receptors (OR) expressed by olfactory sensory neurons (OSN). Odor recognition ...

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