Real-time In Vitro Monitoring of Odorant Receptor Activation by an Odorant in the Vapor Phase
7.0K Views
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09:53 min
•
April 23rd, 2019
DOI :
April 23rd, 2019
•Claire A. de March1, Yosuke Fukutani1,2, Aashutosh Vihani1,3, Hitoshi Kida1,4, Hiroaki Matsunami1,3,5,6
1Department of Molecular Genetics and Microbiology, Duke University Medical Center, 2Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 3Department of Neurobiology, Duke University Medical Center, 4Department of Mechanical Systems, Engineering, Tokyo University of Agriculture and Technology, 5Institute of Global Innovation Research, Tokyo University of Agriculture and Technology, 6Duke Institute for Brain Sciences, Duke University
Chapters in this video
0:04
Title
0:42
Hana3A Cells Culture
2:26
Preparation of the Cells for Transfection
3:23
Plasmid Transfection
5:01
Substrate Incubation
6:07
Odorant Stimulation and Data Analysis
8:07
Results: Real-time Activation and Dose Dependent Responses
9:21
Conclusion
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