Suny Downstate Medical Center

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Real-time Iontophoresis with Tetramethylammonium to Quantify Volume Fraction and Tortuosity of Brain Extracellular Space

John Odackal^*1, Robert Colbourn^*2,3, Namrita Jain Odackal⁴, Lian Tao⁵, Charles Nicholson⁵, Sabina Hrabetova²

¹Department of Medicine, University of Virginia, ²Department of Cell Biology, SUNY Downstate Medical Center, ³Neural and Behavioral Science Graduate Program, SUNY Downstate Medical Center, ⁴Division of Neonatology, University of Virginia, ⁵Department of Neuroscience and Physiology, New York University School of Medicine

This protocol describes real-time iontophoresis, a method that measures physical parameters of the extracellular space (ECS) of living brains. The diffusion of an inert molecule released into the ECS is used to calculate the ECS volume fraction and tortuosity. It is ideal for studying acute reversible changes to brain ECS.

Neuroscience

In Vivo Fiber-Coupled Pre-Clinical Confocal Laser-scanning Endomicroscopy (pCLE) of Hippocampal Capillaries in Awake Mice

Rocio Leal-Campanario^1,2, Susana Martinez-Conde^2,3, Stephen L. Macknik^2,3

¹División de Neurociencias, Universidad Pablo de Olavide, ²Barrow Neurological Institute, ³Ophthalmology, Neurology, and Physiology/Pharmacology, SUNY Downstate Medical Center

Whereas multiphoton imaging is only effective at limited depths from the tissue’s surface, it is possible to achieve 3 µm resolution imaging at any depth via pCLE. Here, we present a protocol to conduct pCLE imaging to measure microvascular dynamics in the hippocampus of ictal and wild-type mice.