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In Situ Characterization of Shewanella oneidensis MR1 Biofilms by SALVI and ToF-SIMS

DOI :

10.3791/55944-v

August 18th, 2017

August 18th, 2017

8,551 Views

1Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, 2Wiley Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory

This article presents a method for growing a biofilm for in situ time-of-flight secondary ion mass spectrometry for chemical mapping in its hydrated state, enabled by a microfluidic reactor, System for Analysis at the liquid Vacuum Interface. The Shewanella oneidensis MR-1 with green fluorescence protein was used as a model.

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SALVI

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