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Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics

DOI :

10.3791/58581-v

10:28 min

October 19th, 2018

October 19th, 2018

20,220 Views

1Université Grenoble Alpes, INRA, CNRS, CEA

Here, we describe a method to purify intact chloroplasts from Arabidopsis leaves and their three main sub-compartments (envelope, stroma, and thylakoids), using a combination of differential centrifugations, continuous Percoll gradients, and discontinuous sucrose gradients. The method is valuable for subplastidial and subcellular localization of proteins by immunoblotting and proteomics.

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Chloroplast Sub compartments

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