Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics
21.0K Views
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10:28 min
•
October 19th, 2018
DOI :
October 19th, 2018
•Chapters in this video
0:04
Title
1:01
Harvesting of Arabidopsis Leaves
2:26
Purification of Crude Chloroplasts Using Differential Centrifugation
3:30
Purification of Intact Chloroplasts on Continuous Percoll Gradient
4:51
Lysis of Intact Chloroplasts Using a Hypotonic Buffer and Purification of Chloroplast Sub-Compartments on Discontinuous Sucrose Gradients
6:05
Washing and Concentration of Thylakoid and Envelope Membrane Systems
7:09
Results: Analysis of Protein Localization by Immunoblotting or Proteomics
8:44
Conclusion
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