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Advanced Confocal Microscopy Techniques to Study Protein-protein Interactions and Kinetics at DNA Lesions

DOI :

10.3791/55999-v

November 12th, 2017

November 12th, 2017

10,662 Views

1Institute of Biophysics of the Czech Academy of Sciences

Laser microirradiation is a useful tool for studies of DNA repair in living cells. A methodological approach for the use of UVA lasers to induce various DNA lesions is shown. We have optimized a method for local microirradiation that maintains the normal cell cycle; thus, irradiated cells proceed through mitosis.

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Confocal Microscopy Techniques

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