JoVE Logo
Faculty Resource Center

Sign In

The MultiBac Protein Complex Production Platform at the EMBL

DOI :

10.3791/50159-v

July 11th, 2013

July 11th, 2013

15,649 Views

1EMBL Grenoble Outstation and Unit of Virus Host Cell Interactions (UVHCI) UMR5322

Protein complexes catalyze key cellular functions. Detailed functional and structural characterization of many essential complexes requires recombinant production. MultiBac is a baculovirus/insect cell system particularly tailored for expressing eukaryotic proteins and their complexes. MultiBac was implemented as an open-access platform, and standard operating procedures developed to maximize its utility.

Tags

MultiBac

-- Views

Related Videos

article

Gross Dissection of the Stomach of the Lobster, Homarus Americanus

article

Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid

article

Using the GELFREE 8100 Fractionation System for Molecular Weight-Based Fractionation with Liquid Phase Recovery

article

Production of Tissue Microarrays, Immunohistochemistry Staining and Digitalization Within the Human Protein Atlas

article

A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells

article

The Logic, Experimental Steps, and Potential of Heterologous Natural Product Biosynthesis Featuring the Complex Antibiotic Erythromycin A Produced Through E. coli

article

Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface

article

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

article

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli

article

CUBIC Protocol Visualizes Protein Expression at Single Cell Resolution in Whole Mount Skin Preparations

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved