Name: isolation of endothelial progenitor cells from human umbilical cord blood
Uploaded: 2017-09-14T14:00:00
Description: Watch this Scientific Journal Video about Isolation of Endothelial Progenitor Cells from Human Umbilical Cord Blood at JoVE.com

Dette materialet er basert på arbeid støttes av National Science Foundation under Grant nr. CMMI-1452943 og ved University of Arkansas utmerkelser College. Vi ønsker også å erkjenne Arkansas Cord Blood Bank for å gi oss med ledningen blod enheter.

denne forskningen ble utført med godkjenning av University of Arkansas institusjonelle Review Board (godkjenningsnummer 16-04-722). Navlestreng blod enheter ble samlet i citrate fosfat druesukker (CPD) løsning på Arkansas Cord Blood Bank, og enheter som ikke oppfyller kravet for lagring ble donert til forskning. Ledningen blod enheter ble sendt med Ilbud til lab innen 24 timer av samlingen ved omgivelsestemperatur.
 1. isolering av endoteliale Progenitor celler fra ledningen blod <ol><li...

<ol>
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C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Redefining+endothelial+progenitor+cells+via+clonal+analysis+and+hematopoietic+stem/progenitor+cell+principals.">Redefining endothelial progenitor cells via clonal analysis and hematopoietic stem/progenitor cell principals.</a> Blood. 109 (5), 1801-1809 (2007).</li><li>Sales, V. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transforming+growth+factor-beta1+modulates+extracellular+matrix+production,+proliferation,+and+apoptosis+of+endothelial+progenitor+cells+in+tissue-engineering+scaffolds.">Transforming growth factor-beta1 modulates extracellular matrix production, proliferation, and apoptosis of endothelial progenitor cells in tissue-engineering scaffolds.</a> Circulation. 114, 193-199 (2006).</li><li>Sales, V. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endothelial+Progenitor+Cells+as+a+Sole+Source+for+Ex+Vivo+Seeding+of+Tissue-Engineered+Heart+Valves.">Endothelial Progenitor Cells as a Sole Source for Ex Vivo Seeding of Tissue-Engineered Heart Valves.</a> Tissue Eng Pt A. 16 (1), 257-267 (2010).</li><li>Liew, A., Barry, F., O'Brien, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endothelial+progenitor+cells:+diagnostic+and+therapeutic+considerations.">Endothelial progenitor cells: diagnostic and therapeutic considerations.</a> Bioessays. 28 (3), 261-270 (2006).</li><li>Hur, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Characterization+of+two+types+of+endothelial+progenitor+cells+and+their+different+contributions+to+neovasculogenesis.">Characterization of two types of endothelial progenitor cells and their different contributions to neovasculogenesis.</a> Arterioscler Thromb Vasc Biol. 24 (2), 288-293 (2004).</li><li>Ingram, D. A., Caplice, N. M., Yoder, M. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Unresolved+questions,+changing+definitions,+and+novel+paradigms+for+defining+endothelial+progenitor+cells.">Unresolved questions, changing definitions, and novel paradigms for defining endothelial progenitor cells.</a> Blood. 106 (5), 1525-1531 (2005).</li><li>Melero-Martin, J. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vivo+vasculogenic+potential+of+human+blood-derived+endothelial+progenitor+cells.">In vivo vasculogenic potential of human blood-derived endothelial progenitor cells.</a> Blood. 109 (11), 4761-4768 (2007).</li><li>Melero-Martin, J. 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H., Varma, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endothelial+progenitor+cells:+what+use+for+the+cardiologist.">Endothelial progenitor cells: what use for the cardiologist.</a> J Angiogenes Res. 2 (6), (2010).</li><li>Camci-Unal, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Surface-modified+hyaluronic+acid+hydrogels+to+capture+endothelial+progenitor+cells.">Surface-modified hyaluronic acid hydrogels to capture endothelial progenitor cells.</a> Soft Matter. 6 (20), 5120-5126 (2010).</li><li>Hill, J. 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L., Szwedo, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Circulating+endothelial+progenitor+cells,+microparticles+and+vascular+disease.">Circulating endothelial progenitor cells, microparticles and vascular disease.</a> J Hypertens. 28 (8), 1611-1613 (2010).</li><li>Nevskaya, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Circulating+endothelial+progenitor+cells+in+systemic+sclerosis+are+related+to+impaired+angiogenesis+and+vascular+disease+manifestations.">Circulating endothelial progenitor cells in systemic sclerosis are related to impaired angiogenesis and vascular disease manifestations.</a> Ann Rheum Dis. 66, 67-67 (2007).</li><li>Cebotari, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Clinical+application+of+tissue+engineered+human+heart+valves+using+autologous+progenitor+cells.">Clinical application of tissue engineered human heart valves using autologous progenitor cells.</a> Circulation. 114, 132-137 (2006).</li><li>Ito, H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endothelial+progenitor+cells+as+putative+targets+for+angiostatin.">Endothelial progenitor cells as putative targets for angiostatin.</a> Cancer Res. 59 (23), 5875-5877 (1999).</li><li>Vasa, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Increase+in+circulating+endothelial+progenitor+cells+by+statin+therapy+in+patients+with+stable+coronary+artery+disease.">Increase in circulating endothelial progenitor cells by statin therapy in patients with stable coronary artery disease.</a> Circulation. 103 (24), 2885-2890 (2001).</li><li>Wu, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tissue-engineered+microvessels+on+three-dimensional+biodegradable+scaffolds+using+human+endothelial+progenitor+cells.">Tissue-engineered microvessels on three-dimensional biodegradable scaffolds using human endothelial progenitor cells.</a> Am J Physiol Heart Circ Physiol. 287 (2), 480-487 (2004).</li><li>Boyer, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Isolation+of+endothelial+cells+and+their+progenitor+cells+from+human+peripheral+blood.">Isolation of endothelial cells and their progenitor cells from human peripheral blood.</a> J Vasc Surg. 31 (1), 181-189 (2000).</li><li>Huber, B., Czaja, A. 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Som nevnt tidligere, tilhenger EPCs har en brosteinsbelagt morfologi. Vår isolerte MNCs kommet fra en spindel-formet celle koloni (figur 2A-2D) i de tidlige stadiene til en brosteinsbelagt koloni (figur 2E-2F) over en periode på ti dager i kultur. EPCs har blitt merket annerledes av forskjellige forskningsgrupper, nemlig som sent endoteliale progenitor celler10, endothelial kolonien danner celler<sup cl...

Flere etterforskere har studert egenskaper og potensialet i menneskelig EPCs5,10,11,12,13. EPCs kan beskrives som sirkulerende celler som har evnen til å overholde endothelial vev i områder av hypoksi, iskemi, skade eller tumor formasjon og bidra til dannelse av nye vaskulære strukturer4,14. De observerte er involvert i neovascularization, i form av postnatal vasculogenesis, har ført til en forståelse av i Patofysiologien ved disse cellene og deres bruk i terapeutiske programmer4,15, 16. antall EPCs i et individ har vist seg å være korrelert med hjerte patologi9,15,16,17,18,19 ,20. Andre studier har også differensiert EPCs i en ventil fibroblast-lignende fenotypen og foreslått at disse cellene kan brukes for tissue engineering hjertet ventiler7,21.
Bestemt celle overflaten molekyler for å isolere EPCs har ikke blitt klart identifisert på grunn av avvik mellom undersøkelser4. Vedheft av MNCs til en bestemt matrise, med eksponering til en rekke kultur forhold, er utført av flere grupper1,17,22,23, antyder at antatte EPCs kan vise ulike fenotypiske egenskaper. Disse egenskapene inkluderer manglende phagocytotic evne, rør formasjon i Matrigel og opptaket av Dil-acetylated low-density lipoproteiner. Den høye clonogenic og proliferativ potensialet er to egenskaper som EPCs kan være hierarchized5. EPCs kan også danne i vitro tubuli når cocultured med menneskelige fosterets lunge fibroblaster4. Disse cellene kalles å uttrykke endothelial celle overflate markører og å dele noen av blodkreft markører13,24,25. Positivt uttrykt markører som er allment akseptert for phenotyping EPCs er CD31, CD34, vaskulær endotelial vekstfaktor reseptor 2 (VEGFR2), von Willebrand faktor (vWF), CD133, c-Kit og vaskulær endotelial cadherin (VE-cadherin)4 , 18. celler som uttrykker co CD90, CD45, CD14, CD115 eller alpha-glatt muskel utgangen (α-SMA) anses ikke å være EPCs på grunn av deres begrensede proliferativ potensial, evne å phagocytose bakterier og manglende evne til å danne de novo menneskelige fartøy i vivo4,7. Denne artikkelen beskriver en endret protokoll for isolering av endoteliale progenitor celler fra menneskelige navlestrengsblod uten behov for en celle sortering protokoller. For denne artikkelen har brukte vi CD31, CD34 og VEGFR2 som positive markører, med α-SMA som negative indikator.
I denne artikkelen foreslår vi en metode for å isolere og dyrking endoteliale progenitor celler fra navlestrengsblod uten celle sortere ved hjelp av spesialiserte endothelial oppblomstringen medium med vekst faktorer (EGF). Denne EGM inneholder vaskulær endotelial vekstfaktor (VEGF) og fibroblast vekstfaktor (FGF), som forbedrer den overlevelse, spredning og migrering av endotelceller26. Det inkluderer også askorbinsyre, som er ansvarlig for å opprettholde brosteinsbelagte morfologi av celler. insulin-lignende vekstfaktor-1 (IGF-1), som gir angiogenic og vandrende funksjon; og heparin, som fører til bedre langsiktig stabilitet av vekstfaktorer i middels26. Andre vekstfaktorer lagt til endothelial celle kultur medium inkluderer tilskudd med epidermal vekstfaktor (EGF), som hjelper i stimulere celle spredning og differensiering hydrocortisone, som sensitizes cellene til EGF26 . Vi viser at bruk av denne bestemte vekstmediet gir høyere antall EPCs sammenlignet endothelial basale medium (EBM) eller Dulbeccos endret Eagle Medium (DMEM).

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			<td height="20" style="width:204px;height:20px;">A) For isolation and culturing</td>
			<td style="width:127px;"></td>
			<td style="width:119px;"></td>
			<td style="width:807px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:204px;height:21px;">EGM-2 BulletKit</td>
			<td style="width:127px;">Lonza</td>
			<td>CC-3162</td>
			<td>This product comes with all the growth factors needed to make the Endothelial Growth Medium</td>
		</tr>
		<tr height="42">
			<td height="42" style="width:204px;height:42px;">Fetal Bovine Serum</td>
			<td style="width:127px;">Thermofisher Scientific</td>
			<td style="width:119px;">26140079</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="width:204px;height:42px;">Pencillin-Streptomycin-Glutamine (100X)</td>
			<td style="width:127px;">Thermofisher Scientific</td>
			<td style="width:119px;">10378016</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:204px;height:21px;">Ficoll-Paque</td>
			<td style="width:127px;">GE Heatlhcare</td>
			<td style="width:119px;">17-1440-02</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="width:204px;height:42px;">Hank&#39;s Balanced Salt Solution</td>
			<td style="width:127px;">Thermofisher Scientific</td>
			<td style="width:119px;">14170-112</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="width:204px;height:42px;">Ammonium Chloride</td>
			<td style="width:127px;">Stem Cell Technologies</td>
			<td style="width:119px;">7850</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="width:204px;height:42px;">1X Phosphate Buffer Saline</td>
			<td style="width:127px;">Thermofisher Scientific</td>
			<td style="width:119px;">14190250</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:204px;height:21px;">Rat Tail I Collagen</td>
			<td style="width:127px;">Corning</td>
			<td style="width:119px;">354236</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:204px;height:21px;">Glacial Acetic Acid</td>
			<td style="width:127px;">Amresco</td>
			<td style="width:119px;">0714-500ML</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="width:204px;height:42px;">0.05% Trypsin-EDTA</td>
			<td style="width:127px;">Thermofisher Scientific</td>
			<td style="width:119px;">25300054</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="width:204px;height:42px;">HEPES buffer</td>
			<td style="width:127px;">Thermofisher Scientific</td>
			<td style="width:119px;">15630080</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="width:204px;height:42px;">Dulbecco&#39;s Modified Eagle&#39;s Medium</td>
			<td style="width:127px;">Thermofisher Scientific</td>
			<td style="width:119px;">10566-016</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:204px;height:21px;"></td>
			<td style="width:127px;"></td>
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			<td height="20" style="width:204px;height:20px;">B) Antibodies and cell lysates</td>
			<td style="width:127px;"></td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:204px;height:21px;">CD31&#160;</td>
			<td style="width:127px;">Abcam</td>
			<td style="width:119px;">ab28364</td>
			<td>1:250 dilution&#160; for Western blotting</td>
		</tr>
		<tr height="42">
			<td height="42" style="width:204px;height:42px;">CD34</td>
			<td style="width:127px;">Santa Cruz Biotechnology</td>
			<td style="width:119px;">sc-7045</td>
			<td>1:100 dilution for Western blotting</td>
		</tr>
		<tr height="21">
			<td height="21" style="width:204px;height:21px;">&#945;-SMA</td>
			<td style="width:127px;">abcam</td>
			<td style="width:119px;">ab5694</td>
			<td>1:100 dilution for Western blotting</td>
		</tr>
		<tr height="21">
			<td height="21" style="width:204px;height:21px;">&#945;-tubulin</td>
			<td style="width:127px;">abcam</td>
			<td style="width:119px;">ab7291</td>
			<td>1:2500 dilution for Western blotting</td>
		</tr>
		<tr height="21">
			<td height="21" style="width:204px;height:21px;">VEGFR2</td>
			<td style="width:127px;">abcam</td>
			<td style="width:119px;">sc504</td>
			<td>1:100 dilution for Western blotting</td>
		</tr>
		<tr height="42">
			<td height="42" style="width:204px;height:42px;">Human umbilical vein endothelial cell lysate</td>
			<td style="width:127px;">Santa Cruz Biotechnology</td>
			<td style="width:119px;">sc24709&#160;</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:204px;height:21px;">Valve interstitial cell lysate</td>
			<td style="width:127px;"></td>
			<td style="width:119px;"></td>
			<td style="width:807px;">Primary cell line cultured from own lab and lysed with RIPA buffer</td>
		</tr>
		<tr height="21">
			<td height="21" style="width:204px;height:21px;"></td>
			<td style="width:127px;"></td>
			<td style="width:119px;"></td>
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			<td height="40" style="width:204px;height:40px;">C) Western blotting and immunostaining</td>
			<td style="width:127px;"></td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:204px;height:21px;">10X Tris/Glycine/SDS buffer</td>
			<td style="width:127px;">Biorad</td>
			<td style="width:119px;">161-0772</td>
			<td>Used as running buffer</td>
		</tr>
		<tr height="21">
			<td height="21" style="width:204px;height:21px;">10X Tris/Glycine buffer</td>
			<td style="width:127px;">Biorad</td>
			<td style="width:119px;">161-0771</td>
			<td>Used as transfer buffer</td>
		</tr>
		<tr height="42">
			<td height="42" style="width:204px;height:42px;">Immobilon-FL transfer membrane</td>
			<td style="width:127px;">Merck Millipore</td>
			<td style="width:119px;">IPFL0010</td>
			<td>This is a PVDF transfer membrane that has 45 &#181;m pore size and is mentioned in the protocol as western blot membrane</td>
		</tr>
		<tr height="21">
			<td height="21" style="width:204px;height:21px;">4X Laemmli sample buffer</td>
			<td style="width:127px;">Biorad</td>
			<td style="width:119px;">161-0747</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:204px;height:21px;">2-mercaptoethanol</td>
			<td style="width:127px;">Biorad</td>
			<td style="width:119px;">161-0710</td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="width:204px;height:21px;">10% Criterion TGX precast gel</td>
			<td style="width:127px;">Biorad</td>
			<td style="width:119px;">5671033</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="width:204px;height:42px;">Prolong Gold antifade</td>
			<td style="width:127px;">Thermofisher Scientific</td>
			<td style="width:119px;">P36930</td>
			<td>Used for mounting immunostained coverslips for long term storage</td>
		</tr>
		<tr height="21">
			<td height="21" style="width:204px;height:21px;">Methanol</td>
			<td style="width:127px;">VWR Analytical</td>
			<td style="width:119px;">BDH1135-4LP</td>
			<td></td>
		</tr>
	</tbody>
</table>

isolation of endothelial progenitor cells from human umbilical cord blood

Eksistensen av endoteliale progenitor celler (EPCs) i perifert blod og dens engasjement i vasculogenesis ble først rapportert av Ashara og kolleger1. Senere dokumentert andre eksistensen av lignende typer EPCs fra benmargen2,3. Flere nylig, Yoder og Ingram viste at EPCs avledet fra navlestrengsblod hadde et høyere proliferativ potensial sammenlignet med de isolert fra voksen perifert blod4,5,6. Bortsett fra å være involvert i postnatal vasculogenesis, har EPCs også vist lovende som en celle for å lage vev-konstruert vaskulær og hjerte ventil konstruksjoner7,8. Ulike isolasjon protokoller finnes, innebære noen som cellen sorteringen av mononukleære celler (MNCs) fra kilder som er nevnt tidligere ved hjelp av endothelial og blodkreft markører eller dyrking disse MNCs med spesialiserte endothelial vekst medium, eller en kombinasjon av disse teknikker9. Her presenterer vi en protokoll for isolasjon og kultur EPCs bruker spesialiserte endothelial medium med vekstfaktorer, uten bruk av immunosorting, etterfulgt av karakterisering av isolerte cellene med vestlige blotting og immunostaining.

Isolasjon og utvidelse av endoteliale Progenitor celler: En skjematisk (figur 1) gis som viser den generelle protokollen. Forskjellige blod komponent lagene ble observert etter tetthet gradert sentrifugering menneskelige navlestreng blod med tetthet gradert medium. På seeding MNCs på kollagen-behandlet plater, ble resultatet av kolonier først observert mellom dager 5 og 7 (figur 2A). Disse ...

Watch this Scientific Journal Video about Isolation of Endothelial Progenitor Cells from Human Umbilical Cord Blood at JoVE.com

Isolation of Endothelial Progenitor Cells from Human Umbilical Cord Blood

Målet med denne protokollen er å isolere endoteliale progenitor celler fra navlestrengsblod. Noen av programmene inkluderer bruk av disse cellene som en biomarkør for å identifisere pasienter med kardiovaskulær risiko, behandle iskemiske sykdommer, og skape vev-konstruert vaskulær og hjerte ventil konstruksjoner.

Isolering av endoteliale Progenitor celler fra menneskelige navlestrengsblod

The existence of endothelial progenitor cells (EPCs) in peripheral blood and its involvement in vasculogenesis was first reported by Ashara and ...

Research

JoVE Journal

Developmental Biology

Direct Induction of Human Neural Stem Cells from Peripheral Blood Hematopoietic Progenitor Cells

Direkte Induction of Human Neural stamceller fra perifert blod Hematopoietiske stamceller

Human disease specific neuronal cultures are essential for generating in vitro models for human neurological diseases. However, the lack of access to ...

Isolation of Neural Stem/Progenitor Cells from the Periventricular Region of the Adult Rat and Human Spinal Cord

Isolering av Neural Stem / stamceller fra periventricular Region of Adult Rat and Human Spinal Cord

Adult rat and human spinal cord neural stem/progenitor cells (NSPCs) cultured in growth factor-enriched medium allows for the proliferation of ...

Isolating Mesangiogenic Progenitor Cells (MPCs) from Human Bone Marrow

Isolating Mesangiogenic Progenitor Cells MPCs from Human Bone Marrow

Isolere Mesangiogenic stamceller (MPCS) fra human benmarg

In a research study aimed to isolate human bone marrow (hBM)-derived Mesenchymal Stromal Cells (MSCs) for clinical applications, we identified a novel ...

Isolation and Characterization of Mesenchymal Stromal Cells from Human Umbilical Cord and Fetal Placenta

Isolering og karakterisering av Mesenchymale stromale celler fra human navlestreng og Fetal Placenta

The human umbilical cord (UC) and placenta are non-invasive, primitive and abundant sources of mesenchymal stromal cells (MSCs) that have increasingly ...

Chondrogenic Pellet Formation from Cord Blood-derived Induced Pluripotent Stem Cells

Kondrogen pelletsdannelse fra Cord Blood-avledede Induced Pluripotent Stamceller

Human articular cartilage lacks the ability to repair itself. Cartilage degeneration is thus treated not by curative but by conservative treatments. ...

Induction of Endothelial Differentiation in Cardiac Progenitor Cells Under Low Serum Conditions

Induksjon av Endothelial differensiering i Cardiac Progenitor celler Under lav Serum forhold

Cardiac progenitor cells (CPCs) may have therapeutic potential for cardiac regeneration after injury. In the adult mammalian heart, intrinsic CPCs are ...

Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term Infants

Isolering og karakterisering av menneskelig navlestreng-avledet Mesenchymal stamceller fra premature og Term spedbarn

Mesenchymal stem cells (MSCs) have considerable therapeutic potential and attract increasing interest in the biomedical field. MSCs are originally ...

Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells

Generasjon 3D huden Organoid fra ledningen blod-avledet indusert Pluripotent stamceller

The skin is the body&#8217;s largest organ and has many functions. The skin acts as a physical barrier and protector of the body and regulates bodily ...

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

I Vitro generasjon av Somite derivater fra menneske indusert stamceller Pluripotent

In response to signals such as WNTs, bone morphogenetic proteins (BMPs), and sonic hedgehog (SHH) secreted from surrounding tissues, somites (SMs) give ...