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A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

DOI :

10.3791/56195-v

10:07 min

August 25th, 2017

August 25th, 2017

7,185 Views

1Gene Editing Institute, Helen F. Graham Cancer Center and Research Institute, Christiana Care Health Services, 2Department of Medical Sciences, University of Delaware

This protocol outlines the workflow of a CRISPR/Cas9-based gene editing system for the repair of point mutations in mammalian cells. Here, we use a combinatorial approach to gene editing with a detailed follow-on experimental strategy for measuring indel formation at the target site—in essence, analyzing onsite mutagenesis.

Tags

Standard Methodology

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