CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy

14.7K Views

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08:22 min

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March 12th, 2018

DOI :

10.3791/56844-v

March 12th, 2018

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Xuan Yao¹^,², Xing Wang¹^,², Junlai Liu²^,³^,⁴, Linyu Shi¹, Pengyu Huang³, Hui Yang¹

¹Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, ²College of Life Sciences, University of Chinese Academy of Sciences, ³School of Life Science and Technology, Shanghai Tech University, ⁴Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences

Summary

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Keywords Extracted From The Text CRISPR Cas9

Targeted Integration

Homology mediated End Joining

Genetic Tool

Genetically Modified Animal Models

Gene Therapies

Therapeutic Potential

Chapters in this video

0:04

Title

0:54

sgRNA Surveyor Nuclease Assay

2:50

Cas9 mRNA Production

4:11

sgRNA Production

5:30

Embryo Collection, Microinjection, and In Vitro Culture

6:43

Results: Superior Integration with Cas9

7:46

Conclusion

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