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Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells

DOI :

10.3791/56274-v

10:48 min

October 30th, 2017

October 30th, 2017

12,184 Views

1Center for Stem Cell Biology and Engineering, University of California, Santa Barbara

This protocol describes how to produce retinal pigment epithelial cells (RPE) from pluripotent stem cells. The method uses a combination of growth factors and small molecules to direct the differentiation of stem cells into immature RPE in fourteen days and mature, functional RPE after three months.

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