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Performing Subretinal Injections in Rodents to Deliver Retinal Pigment Epithelium Cells in Suspension

DOI :

10.3791/52247-v

January 23rd, 2015

January 23rd, 2015

16,801 Views

1Department of Cell and Molecular Biology, The Scripps Research Institute, 2Lowy Medical Research Institute

Here we present a community accepted protocol in multimedia format for subretinally injecting a bolus of RPE cells in rats and mice. This approach can be used for determining rescue potentials, safety profiles, and survival capacities of grafted RPE cells upon implantation in animal models of retinal degeneration.

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Subretinal Injections

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