Microinjection of CRISPR/Cas9 protein into channel catfish, Ictalurus punctatus embryos for gene editing. The development of a reliable and efficient protocol for targeted gene editing in channel catfish, is crucial to studying functional genomics
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En enkel og effektiv microinjection protokoll for genet redigering i kanalen Gråsteinbit embryoer bruker CRISPR/Cas9 systemet er presentert. I denne protokollen, guide RNAs og Cas9 protein var microinjected i eggeplomme av en celle embryo. Denne protokollen er validert av banke ut to kanalen Gråsteinbit immun-relaterte gener.