Cairo University
5 ARTICLES PUBLISHED IN JoVE
Biochemistry
A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
Mohamed Salem 1,2, Michal Bernach 1, Krzysztof Bajdzienko 1, Patrick Giavalisco 1
1Max Planck Institute of Molecular Plant Physiology, Golm, Germany, 2Department of Pharmacognosy, Faculty of Pharmacy, Cairo University
A protocol for comprehensive extraction of lipids, metabolites and proteins from biological tissues using one sample is presented.
JoVE Core
Microinjection of CRISPR/Cas9 Protein into Channel Catfish, Ictalurus punctatus, Embryos for Gene Editing
Ahmed Elaswad *1,2, Karim Khalil *1,3, David Cline 1, Patrick Page-McCaw 4, Wenbiao Chen 4, Maximilian Michel 5, Roger Cone 5, Rex Dunham 1
1School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, 2Department of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University, 3Anatomy and Embryology Department, Faculty of Veterinary Medicine, Cairo University, 4Department of Molecular Physiology and Biophysics, Vanderbilt University, 5Life Science Institute, University of Michigan
A simple and efficient microinjection protocol for gene editing in channel catfish embryos using the CRISPR/Cas9 system is presented. In this protocol, guide RNAs and Cas9 protein were microinjected into the yolk of one-cell embryos. This protocol has been validated by knocking out two channel catfish immune-related genes.
Genetics
Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice
Hirofumi Nishizono *1,2,3, Mohamed Darwish *4,5, Hideki Uosaki 6,7, Nanami Masuyama 8,9,10, Motoaki Seki 8,11, Hiroyuki Abe 3, Nozomu Yachie 8,9,10,12,13, Ryohei Yasuda 1
1Max Planck Florida Institute for Neuroscience, 2Life Science Research Center, University of Toyama, 3Department of Biochemical Engineering, Graduate School of Science and Engineering, Yamagata University, 4Graduate School of Innovative Life Science, University of Toyama, 5Department of Biochemistry, Faculty of Pharmacy, Cairo University, 6Division of Regenerative Medicine, Center for Molecular Medicine, Jichi Medical University, 7Division of Stem Cell Research and Drug Development, Center for Development of Advanced Medical Technology, Jichi Medical University, 8Synthetic Biology Division, Research Center for Advanced Science and Technology, University of Tokyo, 9Institute for Advanced Biosciences, Keio University, 10Graduate School of Media and Governance, Keio University, 11Department of Molecular Oncology, Graduate School of Medicine, Chiba University, 12Department of Biological Sciences, School of Science, University of Tokyo, 13College of Arts and Sciences, University of Tokyo
Here, we present a modified method for cryopreservation of one-cell embryos as well as a protocol that couples the use of freeze-thawed embryos and electroporation for the efficient generation of genetically modified mice.
Chemistry
Synthesis of Black Seed Oil-loaded-, Alginate-based, pH-sensitive Beads Using Electrospraying Technique
Abul Kalam Azad 1, Mohamad Dayoob 2, Vinoth Kumarasamy 3, Vetriselvan Subramaniyan 4, Ghadeer M. Albadrani 5, Muath Q. Al-Ghadi 6, Mohamed Kamel 7, Mohamed M. Abdel-Daim 8,9, Mireia Mallandrich Miret 10, Abd Almonem Doolaanea 11,12
1Faculty of Pharmacy, University College of MAIWP International, 2Faculty of Pharmacy, MAHSA University, 3Department of Parasitology and Medical Entomology, Faculty of Medicine, Universiti Kebangsaan Malaysia, 4Department of Medical Sciences, School of Medical and Life Sciences, Sunway University, 5Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, 6Department of Zoology, College of Science, King Saud University, 7Department of Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Cairo University, 8Department of Pharmaceutical Sciences, Pharmacy Program, Batterjee Medical College, 9Pharmacology Department, Faculty of Veterinary Medicine, Suez Canal University, 10Department of Pharmacy, Pharmaceutical Technology and Physical-Chemistry, Faculty of Pharmacy and Food Sciences, University of Barcelona, 11Research and Development, Sabrena Experience, 12Alphastar Lab Systems
A technique employing high electric voltage and a targeted, active ingredient-loaded emulsion to fabricate pH-responsive, uniform microbeads is presented.
Chemistry
Development and Characterization of Fusidic Acid-Loaded Alginate-Aloe vera Based Hydrogel Film
Mohamad Dayoob 1, Yuki Wong Yee Kee 1, Wan Hamirul Bahrin 2, Bilal Harieth Alrimawi 3, Vinoth Kumarasamy 4, Vetriselvan Subramaniyan 5, Ghadeer M. Albadrani 6, Muath Q. Al-Ghadi 7, Mohamed Kamel 8, Mohamed M. Abdel-Daim 9,10, Abul Kalam Azad 11
1Faculty of Pharmacy, MAHSA University, 2Radiopharmaceutical Technology Group, Medical Technology Division, Malaysia Nuclear Agency, 3Faculty of Pharmacy, Department of Pharmaceutical Sciences, Aqaba University of Technology, 4Department of Parasitology and Medical Entomology, Faculty of Medicine, Universiti Kebangsaan Malaysia, 5Department of Medical Sciences, School of Medical and Life Sciences, Sunway University, 6Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, 7Department of Zoology, College of Science, King Saud University, 8Department of Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Cairo University, 9Department of Pharmaceutical Sciences, Pharmacy Program, Batterjee Medical College, 10Pharmacology Department, Faculty of Veterinary Medicine, Suez Canal University, 11Faculty of Pharmacy, University College of MAIWP International
A formulation of fusidic acid-loaded hydrogel film was developed with different Aloe vera ratios and characterized in this study.
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