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High-resolution Confocal Imaging of the Blood-brain Barrier: Imaging, 3D Reconstruction, and Quantification of Transcytosis

DOI :

10.3791/56407-v

10:30 min

November 16th, 2017

November 16th, 2017

11,100 Views

1Roche Pharma Research and Early Development (pRED), Neuroimmunology, Roche Innovation Center Basel

Here we present a microscopy-based protocol for high-resolution imaging and a three-dimensional reconstruction of the mouse neurovascular unit and blood-brain barrier using brain free-floating sections. This method allows for the visualization, analysis, and quantification of intracellular organelles at the BBB.

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High resolution Confocal Imaging

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