Using CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells
13.0K Views
•
10:21 min
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January 5th, 2018
DOI :
January 5th, 2018
•Chapters in this video
0:05
Title
0:44
Cloning sgRNA Oligos
1:08
Digestion of LentiGuide-puroVector
2:04
Ligation of Annealed Oligos into Digested Backbone
3:02
Generation of Cell Lines Stably Expressing spCas9
5:40
Reporter Assay for Cas9 Activity
6:10
Spinfection of sgRNAs into Cas9 Expressing Cells
6:31
Ba/F3 Cellular Transformation and Positive Selection using m-IL3 Withdrawal
7:23
Results: Use of CRISPR/Cas9 Tool and Ba/F3 Cellular Transformation to Study Oncogenic Potential of Mutated Calreticulin
9:02
Conclusion
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