JoVE Logo
Faculty Resource Center

Sign In

Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA

DOI :

10.3791/56730-v

May 2nd, 2018

May 2nd, 2018

12,019 Views

1Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention

Determining the fungal diversity within an environment is a method utilized in occupational health studies to identify health hazards. This protocol describes DNA extraction from occupational air samples for amplification and sequencing of fungal ITS regions. This approach detects many fungal species that can be overlooked by traditional assessment methods.

Tags

Occupational Air Sample

-- Views

Related Videos

article

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. I. Collection of Virus Samples

article

A Simple Method for Automated Solid Phase Extraction of Water Samples for Immunological Analysis of Small Pollutants

article

Air-sampled Filter Analysis for Endotoxins and DNA Content

article

Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR

article

Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils

article

Modification and Application of a Leaf Blower-vac for Field Sampling of Arthropods

article

Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA

article

Automated, High-resolution Mobile Collection System for the Nitrogen Isotopic Analysis of NOx

article

A Lipid Extraction and Analysis Method for Characterizing Soil Microbes in Experiments with Many Samples

article

Extraction of Organochlorine Pesticides from Plastic Pellets and Plastic Type Analysis

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved