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Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants

DOI :

10.3791/65135-v

May 5th, 2023

May 5th, 2023

1,208 Views

1LabPlaM - Mycoheterotrophic Plants Research Lab, Department of Plant Biology, State University of Campinas (UNICAMP)

The current article aims to provide detailed and adequate protocols for the isolation of plant-associated endophytic fungi, long-term preservation of isolates, morphological characterization, and total DNA extraction for subsequent molecular identification and metagenomic analyses.

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