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A Quantitative Dot Blot Assay for AAV Titration and Its Use for Functional Assessment of the Adeno-associated Virus Assembly-activating Proteins

DOI :

10.3791/56766-v

June 12th, 2018

June 12th, 2018

13,380 Views

1Department of Molecular and Medical Genetics, Oregon Health & Science University, 2Department of Molecular Microbiology and Immunology, Oregon Health & Science University

This manuscript details a straightforward dot blot assay for quantitation of adeno-associated virus (AAV) titers and its application to study the role of assembly-activating proteins (AAPs), a novel class of non-structural viral proteins found in all AAV serotypes, in promoting the assembly of capsids derived from cognate and heterologous AAV serotypes.

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Quantitative

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