A Quantitative Assay for Insulin-expressing Colony-forming Progenitors
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14:39 min
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November 28th, 2011
DOI :
November 28th, 2011
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Chapters in this video
0:05
Title
3:34
Generating Conditioned Medium (CM) For the Colony Assay
5:13
Obtaining a Single Cell Suspension of Pancreatic-like Progenitors by Fluorescence Activated Cell Sorting
7:55
Plating Sorted Cells to Semi-solid Culture
10:49
Observing Colony Formation under the Light Microscope
11:47
Ngn3-EGFP+ Derived Colonies Express Insulin
13:21
Conclusion
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