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Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases

DOI :

10.3791/4000-v

20:21 min

September 1st, 2012

September 1st, 2012

17,163 Views

1Membrane Structural and Functional Biology Group, Schools of Medicine and Biochemistry & Immunology, Trinity College Dublin

Herein is described a robotic approach to high-throughput crystallization of membrane proteins in lipidic mesophases for use in structure determination using macromolecular X-ray crystallography. Three robots capable of handling the viscous and sticky protein-laden mesophase integral to the method are introduced.

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Robot

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