Name: effectiveness of the air stripping in two salmonid fish, rainbow trout (oncorhynchus mykiss) and brown trout (salmo trutta morpha fario)
Uploaded: 2018-09-16T13:00:00
Description: Watch this Scientific Journal Video about Effectiveness of the Air Stripping in Two Salmonid Fish, Rainbow Trout Oncorhynchus Mykiss and Brown Trout Salmo Trutta Morpha fario at JoVE.com

The presented study was supported by Project "Pneumatic method of fish stripping - possible application, influence on the gametes' quality and quantity and the welfare of fish" (acronym: PNEUFISH) financed under "Operational Program Development of the Fisheries Sector and Coastal Areas 2007-2013" (OR-61724-OR1400001/10), funds appropriated to Institute of Animal Reproduction and Food Research Polish Academy of Sciences, Olsztyn, Poland, and support by COST Office (Food and Agriculture COST Action FA1205: AQUAGAMETE). We would like to thank, Stanley Ifeanyi Ugwu for great support in English editing. We want to acknowledge also the animation producers "Studio Filmowe Ruchome Obrazki".

Procedures were carried out in accordance with the Local Committee on the Ethics of Animal Experiments in Olsztyn, Poland.
1.Equipment Preparation
<ol>
	<li>Use a syringe and needle for injection of the gas into the fish body cavity.</li>
	<li>Set the gas pressure at 0.5 bar, and maintain this pressure to be below 1 bar throughout.</li>
	<li>Control the air flow. For the salmonid fish, 1.5 L/min is the most efficient speed of gas exhaustion. Maintain the optimum speed because higher speed mi...

<ol>
	<li>Nelson, J. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Fishes of the World. , (2006).</li><li>Garc&#237;a-Mar&#237;n, J. L., Sanz, N., Pla, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Proportions+of+native+and+introduced+brown+trout+in+adjacent+fished+and+unfished+Spanish+rivers.">Proportions of native and introduced brown trout in adjacent fished and unfished Spanish rivers.</a> Conservation Biology. 12, 313-319 (1998).</li><li>Dietrich, G. J., Wojtczak, M., Slowinska, M., Dobosz, S., Kuzminski, H., Ciereszko, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Broken+eggs+decrease+pH+of+rainbow+trout+(Oncorhynchus+mykiss)+ovarian+fluid.">Broken eggs decrease pH of rainbow trout (Oncorhynchus mykiss) ovarian fluid.</a> Aquaculture. 273, 748-751 (2007).</li><li>Zurbuch, P. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+structure+for+easy+fish+recovery+during+drainage+of+an+impoundment.">A structure for easy fish recovery during drainage of an impoundment.</a> Progressive FishCulturist. 27, 237-238 (1965).</li><li>Cejko, B. I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+different+stripping+methods+of+female+and+activation+medium+on+fertilization+success+in+northern+pike+(Esox+lucius).">Effects of different stripping methods of female and activation medium on fertilization success in northern pike (Esox lucius).</a> Czech Journal of Animal Sciences. 10, 481-486 (2016).</li><li>Morisawa, S., Morisawa, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Induction+of+potential+for+sperm+motility+by+bicarbonate+and+pH+in+rainbow+trout+and+chum+salmon.">Induction of potential for sperm motility by bicarbonate and pH in rainbow trout and chum salmon.</a> Journal of Experimental Biology. 136, 13-22 (1988).</li><li>Billard, R., Cosson, J., Perchec, G., Linhart, O. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Biology+of+sperm+and+artificial+reproduction+in+carp.">Biology of sperm and artificial reproduction in carp.</a> Aquaculture. 129, 95-112 (1995).</li><li>Iuchi, I., Ha, C. R., Sugiyama, H., Nomura, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Analysis+of+chorion+hardening+of+eggs+of+rainbow+trout,+Oncorhynchus+mykiss.">Analysis of chorion hardening of eggs of rainbow trout, Oncorhynchus mykiss.</a> Development Growth and Differentiation. 38, 299-306 (1996).</li><li>Wojtczak, M. A., Kowalski, R. K., Dobosz, S., Goryczko, K., Ku&#378;minski, H., Glogowski, J., Ciereszko, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Assessment+of+water+turbidity+for+evaluation+of+rainbow+trout+(Oncorhynchus+mykiss)+egg+quality.">Assessment of water turbidity for evaluation of rainbow trout (Oncorhynchus mykiss) egg quality.</a> Aquaculture. 242, 617-624 (2004).</li><li>Tabrizi, E. N., Khara, H., Nezami, S. A., Lorestani, R., Shamspour, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Broken+Eggs+Influence+on+Fertilization+Capacity+and+Viability+of+Eggs,+Turbidity+and+pH+of+Ovarian+Fluid+and+Fertilization+Water+in+the+Endangered+Caspian+Brown+Trout,+Salmo+Trutta+Caspius.">Broken Eggs Influence on Fertilization Capacity and Viability of Eggs, Turbidity and pH of Ovarian Fluid and Fertilization Water in the Endangered Caspian Brown Trout, Salmo Trutta Caspius.</a> International Journal of Biology. 3 (1), 161-166 (2011).</li><li>Carl, G. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Beware+of+the+broken+egg!+A+possible+cause+of+heavy+losses+of+salmon+eggs.">Beware of the broken egg! A possible cause of heavy losses of salmon eggs.</a> Progresive Fish-Culturist. 53, 30-31 (1941).</li></ol>

The pneumatic method of egg collection from fish, although more time-consuming than the traditional method (hand stripping), can secure a high quality of matured oocytes (this work). This is related to the low risk of mechanical disruption of the eggs while applying this procedure. High pH of the ovary fluid, as well as an increase in the fertilization rate, attest to the usefulness of the pneumatic method. Overall, the air stripping method of egg collection in fish reproduction could be a promising option in terms of st...

The rainbow trout (Oncorhynchus mykiss) and brown trout (Salmo trutta morpha fario) belong to the Salmonidae family of Salmoniformes1. The production of these two species in global aquaculture has gained rapid growth, owing to their commercial and recreational importance. In Poland, production of salmonid fish stands at around 20 000 tonnes, with rainbow trout being the dominant species. On the other hand, the brown trout represents a major source of freshwater fish resources in Europe because of its commercial value in aquaculture and importance for angling. In many aquatic ecosystems, the existence of brown trout populations is threatened. As a result, artificial reproduction has been applied to restock local populations of this species2.
Rainbow trout and brown trout attain sexual maturity usually at the age of three, with males maturing a year earlier than females1. Under artificial conditions, eggs and sperm of rainbow trout and brown trout are usually collected by gentle abdominal massage. In practice, on a small scale, a hand-stripping method of collecting eggs is cost-effective. However, on a large scale, the hand-stripping method may be labor-intensive and exhausting. As a result, this technique can result in broken eggs due to the weariness of the hatchery workers. Breakage of eggs often leads to a decrease in the pH of ovarian fluid and the release of egg yolk, both of which negatively affect the fertilization of eggs3. Furthermore, this traditional method of egg collection (repeatedly pressing of the abdomen) may also result in broodstock mortality caused by skin infection associated with the deterioration of the protective barrier of mucus. 
Pneumatic method of egg collection was first used in Australia in 19574. The method is based on the injection of gases (air, nitrogen or oxygen) into the body cavity to expel eggs using gas pressure. This fast and easy technique has been successfully applied in salmonid fish without any negative side effects on the fish4. Recently, this method was used in wild species (Northern pike, Esox lucius) to collect mature eggs in artificial conditions5. It was shown that air stripped eggs had higher fertilization and hatching rates in comparison with hand stripped eggs, regardless of the solution used for fertilization (Woynarovich solution, Billard buffer or hatchery water)5.
The principal aim of this study was to test the pneumatic method (air stripping) of collecting eggs in rainbow trout and brown trout in comparison with traditional hand stripping method. Efficiencies of both methods (volume of obtained eggs and time of collection, quality of gametes (ovarian fluid pH), as well as hatching rate and post-spawning mortality) were compared.

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			<td height="21" style="height:21px;width:216px;">Glycine pure P.A.</td>
			<td style="width:215px;">AVANTOR</td>
			<td align="right" style="width:119px;">527560117</td>
			<td style="width:167px;">sperm activating buffer</td>
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			<td height="21" style="height:21px;width:216px;">Trizma base</td>
			<td style="width:215px;">Sigma Aldrich</td>
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			<td>sperm activating buffer</td>
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			<td height="21" style="height:21px;width:216px;">Sodium chloride Bioreagent</td>
			<td style="width:215px;">Sigma Aldrich</td>
			<td style="width:119px;">S5886</td>
			<td>sperm activating buffer</td>
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			<td height="21" style="height:21px;width:216px;">Calcium chloride anhydrous</td>
			<td style="width:215px;">Sigma Aldrich</td>
			<td style="width:119px;">C4901</td>
			<td>sperm activating buffer</td>
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			<td height="42" style="height:42px;width:216px;">Propiscin (0.2% etomidate solution)</td>
			<td style="width:215px;">IRS Olsztyn, Poland</td>
			<td style="width:119px;">not indicated</td>
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			<td height="42" style="height:42px;width:216px;">compressor</td>
			<td style="width:215px;">Thomas&#160; Sheboigan WI USA&#160;</td>
			<td style="width:119px;">DT/SR 070800001882</td>
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			<td height="21" style="height:21px;width:216px;">reduction valve</td>
			<td style="width:215px;">Camozzi&#160;</td>
			<td style="width:119px;">cRJUS U7J</td>
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			<td height="21" style="height:21px;width:216px;">air hose</td>
			<td style="width:215px;">ZEC T.P.U.</td>
			<td style="width:119px;">&#160;SH.98&#160;</td>
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			<td height="21" style="height:21px;width:216px;">syrgine</td>
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			<td align="right">7012118</td>
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			<td height="21" style="height:21px;width:216px;">Air spawning stage</td>
			<td style="width:215px;">Biopasz, Poland</td>
			<td style="width:119px;">PNEU001</td>
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			<td height="42" style="height:42px;width:216px;">Orion Ross Ultra electrode</td>
			<td style="width:215px;">Thermo Scientific, Waltham, MA, USA</td>
			<td style="width:119px;">8102BNUWP</td>
			<td></td>
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effectiveness of the air stripping in two salmonid fish, rainbow trout (oncorhynchus mykiss) and brown trout (salmo trutta morpha fario)

Egg collection is one of the most crucial procedures during fish reproduction in salmonid hatcheries. Classic methods involve the use of hand massage on fish abdomens to expel the eggs. An alternative method uses the pressure of gas injected into the body cavity, which causes the subsequent release of the eggs. This method is believed to have less negative effects on both the welfare and egg quality of the broodstocks. Herein, we compare the results of air and hand stripping methods with respect to one-year survival and egg quantity and quality in two salmonid fish, rainbow trout (Oncorhynchus mykiss) and brown trout (Salmo trutta morpha fario). Our results indicate that air stripping yielded a better quality of eggs and higher one-year survival rate in rainbow trout. In addition, air stripping resulted in lower mortality rate than the group subjected to hand stripping (25% vs. 35%). The pH and hatching rate of the hand stripped group was lower than those of the air stripped group. In the case of brown trout, the quality of eggs obtained by both hand and air-stripping methods was similar; however, the one-year losses in fish were higher in air stripped group (15% compared to 0% in hand stripped fish). Although the advantages of air stripping method over hand stripping in terms of egg quality might not be observed in all salmonid species, the air-stripping procedure might be a promising option to be adopted in hatcheries as it ensures a high level of reproducibility and efficiency.

Mature males and females of rainbow trout (age 2+, 1700 &#177; 328 g) and brown trout (age 3+, 1900 &#177; 435 g) were obtained from the Salmonid Fish Hatchery (Rutki-&#379;ukowo, Inland Fishery Institute in Olsztyn, Poland). The males and females were placed in separate tanks with a volume of about 5 m3. The water temperature was 12&#177;1 &#176;C. Before manipulation, fish were anesthetized with 0.2% Propiscin. The fish were divided into two groups. Part of them ...

Watch this Scientific Journal Video about Effectiveness of the Air Stripping in Two Salmonid Fish, Rainbow Trout Oncorhynchus Mykiss and Brown Trout Salmo Trutta Morpha fario at JoVE.com

Effectiveness of the Air Stripping in Two Salmonid Fish, Rainbow Trout Oncorhynchus Mykiss and Brown Trout Salmo Trutta Morpha fario

The principal aim of this study is to standardize and test the pneumatic method (air stripping) of collecting eggs in rainbow trout and brown trout. This method allows effective and simple collection of the eggs without the necessity of fish abdomen massage.

Effectiveness of the Air Stripping in Two Salmonid Fish, Rainbow Trout (Oncorhynchus Mykiss) and Brown Trout (Salmo Trutta Morpha fario)

Egg collection is one of the most crucial procedures during fish reproduction in salmonid hatcheries. Classic methods involve the use of hand massage on ...

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