May 28th, 2015
•Genome editing tools such as the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas (CRISPR-associated) system have greatly improved gene targeting efficiency in human induced pluripotent stem cells (hiPSCs). This manuscript describes a protocol for generating lineage specific hiPSC reporter using CRISPR/Cas system assisted homologous recombination.
Related Videos
Alginate Encapsulation of Pluripotent Stem Cells Using a Co-axial Nozzle (Video) | JoVE
Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of Neural Stem Cell Autonomous and Non-autonomous Effects (Video) | JoVE
A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice (Video) | JoVE
Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells (Video) | JoVE
Culturing Human Pluripotent and Neural Stem Cells in an Enclosed Cell Culture System for Basic and Preclinical Research (Video) | JoVE
Co-localization of Cell Lineage Markers and the Tomato Signal (Video) | JoVE
Cell Lineage Analyses and Gene Function Studies Using Twin-spot MARCM (Video) | JoVE
CRISPR/Cas9 Technology in Restoring Dystrophin Expression in iPSC-Derived Muscle Progenitors (Video) | JoVE
Three-dimensional Angiogenesis Assay System using Co-culture Spheroids Formed by Endothelial Colony Forming Cells and Mesenchymal Stem Cells (Video) | JoVE
Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells (Video) | JoVE
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved