JoVE Logo
Faculty Resource Center

Sign In

Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence

DOI :

10.3791/58321-v

9:36 min

November 6th, 2018

November 6th, 2018

8,384 Views

1Graduate Program in Pharmacology and Experimental Therapeutics, University of Cologne, 2Department of Dermatology and Venereology, University Hospital of Cologne, 3Cologne Cluster of Excellence in Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, 4Department of General Ecology, Institute for Zoology, Biocenter Cologne, University of Cologne

The node and notochordal plate are transient signaling organizers in developing mouse embryos that can be visualized using several techniques. Here, we describe in detail how to perform two of the techniques to study their structure and morphogenesis: 1) scanning electron microscopy (SEM); and 2) whole mount immunofluorescence (WMIF).

Tags

Node

-- Views

Related Videos

article

Contrast Imaging in Mouse Embryos Using High-frequency Ultrasound

article

Large-scale Scanning Transmission Electron Microscopy (Nanotomy) of Healthy and Injured Zebrafish Brain

article

Multi-target Chromogenic Whole-mount In Situ Hybridization for Comparing Gene Expression Domains in Drosophila Embryos

article

Methods to Examine the Lymph Gland and Hemocytes in Drosophila Larvae

article

Organ Culture and Whole Mount Immunofluorescence Staining of Mouse Wolffian Ducts

article

Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence

article

Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos

article

Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes

article

Whole Mount Immunofluorescence and Follicle Quantification of Cultured Mouse Ovaries

article

Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved