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Microprobe Capillary Electrophoresis Mass Spectrometry for Single-cell Metabolomics in Live Frog (Xenopus laevis) Embryos

DOI :

10.3791/56956-v

December 22nd, 2017

December 22nd, 2017

18,951 Views

1Department of Chemistry, George Washington University, 2Department of Anatomy & Regenerative Biology, George Washington University, 3Department of Chemistry & Biochemistry, University of Maryland, College Park

We describe steps that enable fast in situ sampling of a small portion of an individual cell with high precision and minimal invasion using capillary-based micro-sampling, to facilitate chemical characterization of a snapshot of metabolic activity in live embryos using a custom-built single cell capillary electrophoresis and mass spectrometry platform.

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