Standardized Measurement of Nasal Membrane Transepithelial Potential Difference (NPD)

16.1K Views

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09:47 min

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September 13th, 2018

DOI :

10.3791/57006-v

September 13th, 2018

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George M. Solomon¹, Inez Bronsveld², Kathryn Hayes³, Michael Wilschanski⁴, Paola Melotti⁵, Steven M. Rowe¹, Isabelle Sermet-Gaudelus⁶^,⁷

¹Department of Medicine and the Gregory Fleming James Cystic Fibrosis Center, University of Alabama at Birmingham, ²Department of Pulmonology and Tuberculosis, University Medical Center Utrecht, ³Center for Experimental Medicine, Queens University, Northern Ireland, ⁴Hadassah Hebrew University Medical Center, Jerusalem, ⁵Centro Fibrosi Cistica, Azienda Ospedaliera Universitaria Integrata, ⁶Service de Pneumologie et Allergologie Pédiatriques and Center de Ressources et de Compétence de la Mucoviscidose, Hôpital Necker Enfants Malades, ⁷INSERM U 1151, Institut Necker Enfants Malades

Summary

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Keywords Nasal Potential Difference

NPD

Cystic Fibrosis Transmembrane Conductance Regulator

CFTR

Epithelial Sodium Channel

Chapters in this video

0:04

Title

1:01

Materials Setup

2:19

Measurement Electrode Placement

4:35

Basal Potential Difference (PD) Measurement

6:02

Nasal Membrane Transepithelial Potential Difference (NPD) Tracing Sequential Perfusions

7:42

Results: Representative CF and Healthy Subject Tracings

8:57

Conclusion

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