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DOI :
10.3791/57061-v
December 9th, 2017
Chapters
0:00
Title
0:09
Introduction
1:03
Protocol
1:07
Introducing the Stop Codon into the Target Gene
2:58
Expressing and Purifying Site-specifically Acetylated Protein
6:21
Biochemically Characterizing Acetylated Protein
9:39
Results
10:37
Summary
基因编码扩展是研究广泛的生物过程, 包括蛋白质乙酰化的有力工具。在这里, 我们演示了一个轻便的协议, 利用这种技术, 在特定的地点, 在大肠杆菌细胞生成均匀乙酰蛋白。
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