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DOI :
10.3791/57061-v
December 9th, 2017
Chapters
0:00
Title
0:09
Introduction
1:03
Protocol
1:07
Introducing the Stop Codon into the Target Gene
2:58
Expressing and Purifying Site-specifically Acetylated Protein
6:21
Biochemically Characterizing Acetylated Protein
9:39
Results
10:37
Summary
遺伝コードの拡張は、タンパク質アセチル化を含む、生物学的プロセスの広い範囲を研究するための強力なツールとして機能します。均一に生成するためのこの手法を悪用する安易なプロトコルをアセチル化したタンパク質の大腸菌細胞の特定のサイトを示します。
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