Villous Tree Dissection and Immunofluorescent Staining
3:16
Clarification of the Tissue
4:07
Mounting for Microscopy and Confocal Microscopy
6:04
Reversing the Clearing
6:33
Results: Use of Confocal Microscopy, Immunostaining, and Reverse Clearing to Analyze the Vascularization of Placental Network
8:21
Conclusion
Transcript
The overall goal of this procedure is to generate three-D renderings of placental villous tree fragments that are suitable for the analysis of their contained vascular networks. This method can help us answer key questions in our field, such as wh
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This study presents a protocol for the reversible tissue clearing, immunostaining, 3D-rendering and analysis of vascular networks in human placenta villi samples on the order of 1 - 2 mm3.