JoVE Logo
Faculty Resource Center

Sign In

Two-photon Intravital Imaging of Leukocytes During the Immune Response in Lipopolysaccharide-treated Mouse Liver

DOI :

10.3791/57191-v

February 6th, 2018

February 6th, 2018

9,854 Views

1Department of Anatomy, Yonsei University College of Medicine, 2Brain Korea 21 Plus Project for Medical Science, Yonsei University College of Medicine, 3Department of Surgery, Yonsei University College of Medicine

We established a novel surgical protocol for two-photon imaging of live mice liver with minimal invasion. With this technique, we identified the detailed structure of the liver during lipopolysaccharide-induced endotoxemia. We anticipate that this method may be utilized to determine the effectiveness of various reagents treatment to hepatic leukocyte migration.

Tags

Keywords Two photon Intravital Imaging

-- Views

Related Videos

article

An Introduction to Parasitic Wasps of Drosophila and the Antiparasite Immune Response

article

Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy

article

Intravital Imaging of the Mouse Popliteal Lymph Node

article

Quantitative Measurement of the Immune Response and Sleep in Drosophila

article

Quantification of the Respiratory Burst Response as an Indicator of Innate Immune Health in Zebrafish

article

Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages

article

In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection

article

Intravital Microscopy Imaging of the Liver following Leishmania Infection: An Assessment of Hepatic Hemodynamics

article

Long Term Intravital Multiphoton Microscopy Imaging of Immune Cells in Healthy and Diseased Liver Using CXCR6.Gfp Reporter Mice

article

Non-invasive Imaging of the Innate Immune Response in a Zebrafish Larval Model of Streptococcus iniae Infection

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved