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In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection

DOI :

10.3791/52121-v

November 27th, 2014

November 27th, 2014

8,073 Views

1Department of Microbiology & Immunology, The University of Texas Medical Branch, 2Department of Pathology, The University of Texas Medical Branch, 3Center for Biodefense and Emerging Infectious Diseases, Sealy Center for Vaccine Development, The University of Texas Medical Branch

Two flow cytometry-based methods – an in vitro T cell priming assay and intracellular cytokine staining were utilized to measure antigen presenting capacity of dendritic cells and antigen-specific T cell responses to a West Nile virus mutant infection in mice.

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In Vitro Analysis

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