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Intravital Imaging of the Mouse Popliteal Lymph Node

DOI :

10.3791/3720-v

8:41 min

February 8th, 2012

February 8th, 2012

27,444 Views

1Department of Pediatrics, Case Western Reserve University , 2Department of Pediatrics, Pathology and Biomedical Engineering, Case Western Reserve University

Recent advances in 2-photon microscopy have enabled real-time in situ imaging of live tissues in animal models, thereby enhancing our ability to investigate cellular behavior in both physiologic and pathologic conditions. Here, we outline the preparations required to perform intravital imaging of the mouse popliteal lymph node.

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Intravital Imaging

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