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Rare Event Detection Using Error-corrected DNA and RNA Sequencing

DOI :

10.3791/57509-v

10:36 min

August 3rd, 2018

August 3rd, 2018

11,415 Views

1Department of Pediatrics, Division of Hematology and Oncology, Washington University School of Medicine, 2Center for Genome Sciences and Systems Biology, Washington University School of Medicine

Next-generation sequencing (NGS) is a powerful tool for genomic characterization that is limited by the high error rate of the platform (~0.5–2.0%). We describe our methods of error-corrected sequencing that allow us to obviate the NGS error rate and detect mutations at variant allele fractions as rare as 0.0001.

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Rare Event Detection

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