Single Cell Suspension Quality Control and Single Cell Partitioning
4:10
Single Cell cDNA Amplification
6:19
Library Sequencing
8:11
Read Alignment and Data Analysis
9:53
Results: Dissociation, Single-cell Emulsion, cDNA Library, and Cell Types
11:09
Conclusion
Transcript
This protocol generates high throughput transcription data of individual human islet cells, which can be used to study anochrome cell heterogeneity, identify rare cell type, and gene regulation in disease. This technique generates larger scale sin
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Here, we present a protocol to generate high-quality, large-scale transcriptome data of single cells from isolated human pancreatic islets using a droplet-based microfluidic single-cell RNA sequencing technology.