JoVE Logo
Faculty Resource Center

Sign In

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens

DOI :

10.3791/57471-v

May 5th, 2018

May 5th, 2018

9,112 Views

1Department of Research, Hackensack University Medical Center, 2Department of Medical Sciences, Seton Hall University, 3Department of Epidemiology and Population Health, Albert Einstein College of Medicine, 4Department of Nephrology and Hypertension, Hadassah - Hebrew University Medical Center

Formalin-fixed paraffin-embedded specimens represent a valuable source of molecular biomarkers of human diseases. Here we present a laboratory-based cDNA library preparation protocol, initially designed with fresh frozen RNA, and optimized for the analysis of archived microRNAs from tissues stored up to 35 years.

Tags

Retrospective MicroRNA Sequencing

-- Views

Related Videos

article

Methyl-binding DNA capture Sequencing for Patient Tissues

article

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

article

Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA

article

A Universal Protocol for Large-scale gRNA Library Production from any DNA Source

article

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

article

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

article

Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen

article

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

article

Single-cell RNA Sequencing and Analysis of Human Pancreatic Islets

article

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved