Induction of Germination for Respiration Monitoring and Obtaining Oxygen Consumption Rate (OCR) Measurements
2:34
Induction of Teliospore Germination to Isolate Teliospores at Distinct Stages of Germination and Preparation of Micromanipulator
4:34
Isolation of Stage-specific Germinating Teliospores
6:01
Results: Microrespiration Analysis and Microdissection for Assessing Teliospore Germination
7:32
Conclusion
Transcript
The overall goal of the procedures presented here is to identify stages of spore germination so that gene transcript levels can be assessed. Analysis of these changes will provide further knowledge of a critical stage in disease dispersal. This me
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Smut fungi cause many devastating agricultural diseases. They are dispersed as dormant teliospores that germinate in response to environmental cues. We outline two methods to investigate molecular changes during germination: measuring respiration increase to detect metabolic activation and assessing changing molecular events by isolating teliospores at distinct morphological stages.