Performing DNA Hybridization on the Surface of Polyacrylamide Oligomicrosphere
7:06
Microsphere-PCR for Amplifying ssDNA
8:08
Confocal Microscopy Acquisition
8:38
"Results: Fabrication of Droplet-base Microfluidic Platform and Application of Microspheres for Microsphere-PCR Amplification"
10:33
Conclusion
Transcript
This method can help answer key questions in the combinatorial technique of the microfluidic system and the molecular biotechnology field, such as microfluidic devices and asymmetry PCR method. The main advantages of this technique are efficient g
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This work provides a method for the fabrication of droplet-based microfluidic platforms and the application of polyacrylamide microspheres for microsphere-PCR amplification. The microsphere-PCR method makes it possible to obtain single-stranded DNA amplicons without separating double-stranded DNA.