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Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

9.3K Views

09:30 min

August 6th, 2018

DOI :

10.3791/57902-v

August 6th, 2018


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Protein Complexes

Chapters in this video

0:04

Title

1:28

Assembly of Prepore Anthrax Toxin Complex on a PDEA-modified Amine Reactive Biosensor Surface

4:37

Assembly of Pore Anthrax Toxin Complex on PDEA-modified Amine Reactive Biosensor Surface

5:26

Identification of Released Macromolecular Assemblies from BLI Biosensors by Negative Stain Electron Microscopy and Mass Spectrometry

6:50

Results: Analysis of Dynamic Protein Complexes by Biolayer Interferometry, Electron Microscopy, and Mass Spectrometry

8:29

Conclusion

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