Functional Evaluation of Excitation-contraction-coupling
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Results: Staining of Rat ACMs with Fluo4-AM
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Conclusion
Transcript
This method can help answer key questions in the field of individual cardiomyocyte function and allows studying excitation-contraction-coupling. The main advantage of this technique is that a high yield of viable atrial cardiomyocytes from hearts
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Here, we describe an optimized, Langendorff-based procedure for the isolation of single-cell atrial cardiomyocytes from a rat model of metabolic syndrome-related heart failure with preserved ejection fraction. A manual regulation of intraluminal pressure of cardiac cavities is implemented to yield functionally intact myocytes suitable for excitation-contraction-coupling studies.