JoVE Logo
Faculty Resource Center

Sign In

The Inverted Heart Model for Interstitial Transudate Collection from the Isolated Rat Heart

DOI :

10.3791/55849-v

June 20th, 2017

June 20th, 2017

8,256 Views

1Department of Anesthesiology, Changhai Hospital, Second Military Medical University, 2Department of Molecular Cardiology, Heinrich Heine University, 3Department of Clinical Biochemistry and Pathobiochemistry, German Diabetes Center, Heinrich Heine University

This protocol describes a method to collect cardiac interstitial fluid from the isolated, perfused rat heart. To physically separate interstitial transudate from coronary venous effluent perfusate, the Langendorff perfused heart is inverted, and the transudate (interstitial fluid) formed on the cardiac surface is collected using a soft latex cap.

Tags

Inverted Heart Model

-- Views

Related Videos

article

Orthotopic Aortic Transplantation: A Rat Model to Study the Development of Chronic Vasculopathy

article

A Murine Model of Myocardial Ischemia-reperfusion Injury through Ligation of the Left Anterior Descending Artery

article

Implantation of Total Artificial Heart in Congenital Heart Disease

article

An Isolated Working Heart System for Large Animal Models

article

Method of Isolated Ex Vivo Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program

article

Murine Isolated Heart Model of Myocardial Stunning Associated with Cardioplegic Arrest

article

Repeated Blood Collection from Tail Vein of Non-Anesthetized Rats with a Vacuum Blood Collection System

article

A Cryoinjury Model to Study Myocardial Infarction in the Mouse

article

An Image Guided Transapical Mitral Valve Leaflet Puncture Model of Controlled Volume Overload from Mitral Regurgitation in the Rat

article

Blood Circuit Reconstruction in an Abdominal Mouse Heart Transplantation Model

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved