Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

13.0K Views

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08:23 min

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September 25th, 2018

DOI :

10.3791/58051-v

September 25th, 2018

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Charles Decraene¹^,², Amanda Bortolini Silveira¹, Marc Michel¹, François-Clément Bidard¹^,³^,⁴, Jean-Yves Pierga¹^,³^,⁵, Marc-Henri Stern¹^,⁶, Charlotte Proudhon¹

¹Circulating Tumor Biomarkers Laboratory, SiRIC, Translational Research Department, Institut Curie, PSL Research University, ²CNRS UMR144, Institut Curie, PSL Research University, ³Department of Medical Oncology, Institut Curie, PSL Research University, ⁴University Versailles Saint-Quentin-en-Yvelines, ⁵University Paris Descartes, ⁶Inserm U830, Institut Curie, PSL Research University

Summary

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Keywords Single Droplet Digital PCR

Circulating Tumor DNA

Mutation Detection

Hotspot Regions

Cancer Diagnosis

Absolute Quantification

Chapters in this video

0:04

Title

0:48

Blood Collection and Plasma Storage

2:04

cfDNA Extraction

4:45

ddPCR Mix Preparation

5:46

Droplet Generation

6:25

PCR Amplification

7:06

Results: KRAS and EGFR Drop-off ddPCR Assays

7:50

Conclusion

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