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Functional Characterization of Carboxylesterases in Insecticide Resistant House Flies, Musca Domestica

DOI :

10.3791/58106-v

August 23rd, 2018

August 23rd, 2018

7,198 Views

1Department of Entomology and Plant Pathology, Auburn University

Here, we present a protocol to produce house fly carboxylesterase proteins in vitro with a baculovirus mediated insect cell expression system and later functionally characterize their roles in metabolizing permethrin, thereby, conferring pyrethroid resistance by conducting cell-based MTT assay and in vitro metabolic studies.

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