<h2 class="jove_title"><a href="https://www.jove.com/video/54803">A Method for Extracting Pigments from Squid Doryteuthis pealeii</a></h2>Christopher W. DiBona1, Thomas L. Williams1, Sean R. Dinneen1, Stephanie F. Jones Labadie1, Leila F. Deravi1,21Department of Chemistry, University of New Hampshire, 2Materials Science Program, University of New HampshireA protocol for the extraction of pigments from the nanostructured granules in squid Doryteuthis pealeii chromatophores is presented.<h2 class="jove_title"><a href="https://www.jove.com/video/54611">Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds</a></h2>Michael Joe Vaughan1,2, Ann Chanon3, Joshua J. Blakeslee1,3,41Center for Applied Plant Sciences, The Ohio State University, OARDC, 2Department of Plant Pathology, The Ohio State University, OARDC, 3Department of Horticulture and Crop Science, The Ohio State University, OARDC, 4OARDC Metabolite Analysis Cluster, The Ohio State University, OARDCThis article presents a method for the detection and quantification of organic acids from plant material using free zonal capillary electrophoresis. An example of the potential application of this method, determining the effects of a secondary fermentation on organic acid levels in coffee seeds, is provided.<h2 class="jove_title"><a href="https://www.jove.com/video/54790">Using Pharmacological Manipulation and High-precision Radio Telemetry to Study the Spatial Cognition in Free-ranging Animals</a></h2>Timothy C. Roth*1, Aaron R. Krochmal*2, William B. Gerwig, IV1, Sage Rush3, Nathaniel T. Simmons2, Jeffery D. Sullivan4, Katrina Wachter51Department of Psychology, Franklin and Marshall College, 2Department of Biology, Washington College, 3University of Pennsylvania, 4School of Forestry and Wildlife Sciences, Auburn University, 5Morsani College of Medicine, University of South FloridaThis paper describes a novel protocol that combines the pharmacological manipulation of memory and radio telemetry to document and quantify the role of cognition in navigation.<h2 class="jove_title"><a href="https://www.jove.com/video/52895">Characterization of Metabolic Status in Nonhuman Primates with the Intravenous Glucose Tolerance Test</a></h2>Michael Staup, George Aoyagi, TeQuana Bayless, Yixin Wang, Keefe ChngCardiovascular and Metabolism Group, Crown BioscienceThe goal of this protocol is to present a standard method to perform intravenous glucose tolerance tests (IVGTTs) to assess glycemic control in nonhuman primatesand assess their metabolic status from healthy to dysmetabolic.

november 2016 - this month in jove: turtle tracking, acids in coffee, squid coloration and glucose tolerance in primates

Here's a look at what's coming up in the November 2016 issue of <a href="http://www.jove.com/">JoVE: The Journal of Visualized Experiments</a>.
Think you're good at navigation? How about if you had no access to a GPS, and were also a turtle? In <a href="http://www.jove.com/behavior">JoVE Behavior</a> this month, <a href="http://www.jove.com/video/54790/using-pharmacological-manipulation-high-precision-radio-telemetry-to">Roth et al.</a> (our authors) investigated the role of cognition in the navigation strategies of Eastern painted turtles. After administering a drug that manipulates the cognitive spatial abilities of the animals, the team tracked radio-tagged turtles moving on familiar routes between seasonal water sources. The results showed that the adult animals use a combination of spatial memory and recall to find their pools rather than solely environmental cues...and they didn't use their cellphones once.
Whether you're mocha mad, cappuccino crazy, an espresso purist, or down with the pumpkin spice, coffee is arguably the ultimate crossover of comfort and fuel. Too many short chain carboxylic acids in your cup of joe, however, can turn your morning sour. In <a href="http://www.jove.com/chem">Jove Chemistry</a> this month, <a href="http://www.jove.com/video/54611/using-capillary-electrophoresis-to-quantify-organic-acids-from-plant">Vaughan et al.</a> (our authors) demonstrate methods to safely and rapidly evaluate organic acid levels in food and beverage samples. Using Coffea arabica seeds as an example, the team showed that capillary electrophoresis could identify organic acids to a low limit of detection, and at much lower cost than traditional methods. It's great news for a drink that is perhaps even as vital to the productivity of labs around the world as <a href="http://www.jove.com/">JoVE</a>.
Our journey so far has taken us from terrestrial ponds, then back to land, and now we head out to sea for the subject of our third video spotlight this month...the brilliant and cryptic squid. Cephalopods can undergo rapid color changes, as a response to their environment, for communication, or for sensing. In <a href="http://www.jove.com/biology">JoVE Biology</a>, <a href="http://www.jove.com/video/54803/a-method-for-extracting-pigments-from-squid-doryteuthis-pealeii">DiBona et al.</a> (our authors) reveal a new method for isolating the nanostructured pigment granules from the colorful chromatophores of these oceanic enigmas. Using a combination of simple dissection, sonication, and acidic methanol extraction, the team shows how the soluble pigments can then be extracted from these granules, allowing detailed examination of the individual components that facilitate the dynamic mechanism of adaptive coloration in cephalopods.
Finally this month, we turn our attention to the all-encompassing topic of health. In <a href="http://www.jove.com/med">JoVE Medicine</a>, <a href="http://www.jove.com/video/52895/characterization-metabolic-status-nonhuman-primates-with-intravenous">Staup et al.</a> (our authors) investigate the use of the intravenous glucose tolerance test, or IVGTT, to characterize the metabolic condition of large colonies of non-human primates. A convenient and simple assay that is routinely used in humans, the authors demonstrate that the IVGTT can also be used in macaques to monitor the progression of animals from healthy, toward overt diabetes. Amongst other uses such as studying metabolic disease, this research can help to inform animal care and health management.
You've just had a sneak peek at the November 2016 issue of <a href="http://www.jove.com/">JoVE</a>. Visit the website to see the full-length articles, plus many more, in <a href="http://www.jove.com/">JoVE: The Journal of Visualized Experiments</a>.

Watch this Scientific Journal Video about November 2016 - This Month in JoVE: Turtle tracking, acids in coffee, squid coloration and glucose tolerance in primates at JoVE.com

November 2016 - This Month in JoVE: Turtle tracking, acids in coffee, squid coloration and glucose tolerance in primates

Here's a look at what's coming up in the November 2016 issue of JoVE: The Journal of Visualized Experiments.
Think you're good at navigation? How about if ...

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1.8K Views.  JoVE Content Production. November 2016 - This Month in JoVE: Turtle tracking, acids in coffee, squid coloration and glucose tolerance in primates

Video: November 2016 - This Month in JoVE: Turtle tracking, acids in coffee, squid coloration and glucose tolerance in primates

August 2012: This Month in JoVE

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and mirrors to refract and direct light to optical sensors. The August 2012 issue of JoVE (Journal of Visualized Experiments) is marked by the third publication from the Ozcan Lab (University of California, Los Angeles) on their lens free lens-free "on-chip" microscopy platform, which they have pioneered.
Back in 2009, the Ozcan lab published their first article, which demonstrated the use of a lens-free imaging platform that could image cells within whole blood. This imaging technique involves placing a sample directly on an optoelectric sensor array, like the CCD or CMOS chips found in the standard camcorder. Then, LED illumination from above the sample casts shadows on the chip, which are recorded as digital holograms that can be subjected to image processing. Not only does this lensless imaging system allow for an extremely large field of view (FOV of 0.6-8 cm2), the lack of bulky optical components makes it excellent for microfluidic applications. The Ozcan group demonstrates this in their follow-up 2011 JoVE article, in which fluorescently labeled white blood cells are imaged within a microfluidic chip.
This August in JoVE, the Ozcan group demonstrates further advancements to their lens-free microscopy platform by applying it to tomographic imaging of the nematode, C. elegans. By changing the angle of illumination, using a motorized rotation stage, the Ozcan group shows how multiple holographic slices may be generated for a sample-which, following processing, can be combined into tomograms. The Ozcan group confirms that their 3D reconstruction of the worm is successful by citing that the pharyngeal tube, a 5-micron-thick structure, is only visible in a single slice and in a 3D reconstructed cross section.
The compact and inexpensive nature of the lens-free imaging systems - designed and assembled by the Ozcan group - make them well suited for point-of-care diagnostic applications, or even cell phone based microscopy that can be performed in the field.
At the Ernest Gallo Clinic and Research Center at University of California, San Francisco JoVE learns how to visualize morphological changes in single cells using three-dimensional fluorescence microscopy and computer analysis. In this procedure, cells are given agonist, antagonist plus agonist, or no treatment, and prepared for immunofluorescence microscopy. Three-dimensional images are acquired with the help of a two-photon laser system, and the fluorescence data are processed on the computer using Imaris and Matlab.
To demonstrate the power of this technique, our authors quantify single-cell phenotypic changes in transfected HEK293 cells. Three-dimensional morphometric analysis shows dramatic changes in cells treated with agonist. These changes are not always apparent when using conventional 2D analysis. This technique allows morphological changes in single cells to be quantified and characterized in response to pharmacologic stimuli, offering a powerful new assessment tool to the arsenal of drug discovery research.
This month, in JoVE Applied Physics, we travel to South Carolina to visit the School of Materials Science and Engineering at Clemson University. Here, researchers show us how to construct lithium batteries for laboratory research.
The basic components of a lithium coin cell are the working electrode, the lithium electrolyte, and a lithium foil counter electrode. The different components of the coin cell are carefully assembled and sealed with a crimping machine. The finished coin cell can then be characterized in a battery tester. Using their homemade coin cells, our researchers can experiment with using different materials to make the cathodes of lithium coin cells.
This month in JoVE Immunology and Infection, we head to Basel, Switzerland to witness two techniques for diagnosing helminth infections. We also see how investigators perform the shuttle run test in children, in order to assess the effects of these infections on physical fitness.
Helminths are parasitic worms that can live inside humans and animals. Many species infect the intestinal tract, and may be transmitted through soil. Our authors demonstrate the Kato-Katz technique, which is used for diagnosing several common soil-transmitted helminths. They also demonstrate the Baermann technique, which uses light to stimulate the migration of certain types of phototactic helminths out of sample preparations. The various types of helminth species can subsequently be determined based on morphology.
Our authors then demonstrate how to set up and perform a 20-meter shuttle run test for children, and to measure the impact of helminth infections on physical fitness. This method can help determine the true burden of soil-transmitted helminth infections on overall health.
This brief summary highlights but a few notable video-articles that will be released in August. We'll also feature articles that demonstrate how to express genes in the midbrain of the chick embryo, study neutrophil transmigration in parallel flow chambers lined with endothelial cells, and precisely measure proteolytic degradation of the extracellular matrix.

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and mirrors to refract and direct light to optical sensors. The August 2012 issue of JoVE (Journal of Visualized Experiments) is marked by the third publication from the Ozcan Lab (University of California, Los Angeles) on their lens-free "on-chip" microscopy platform, which they have pioneered.

Traditional microscopy requires lens objectives to magnify specimens, and can involve numerous optical components like additional objectives, filters, and ...

Research

JoVE Journal

Biology

2012: A Year In Review

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE reached a major milestone by publishing our 1500th article. In this publication, in JoVE Bioengineering, Yu et al. described a method for analyzing the free radical composition of cigarette smoke. This method simulated cigarette puffing with a single-port smoking device, and combined it with electron spin resonance (ESR) spectroscopy to measure the degree to which antioxidant compounds can scavenge free radicals in cigarette smoke. A possible outcome of this research is the creation of less harmful cigarettes.
JoVE Bioengineering also contained the most-viewed article of 2012. Owczarczak et al. described a method for hacking a standard inkjet printer, loading ink cartridges with a cell-suspension "bioink," and printing lines of live cells onto glass slides. The thermal inkjet printing process creates temporary pores in the cell membrane, visualized by the incorporation of fluorescently labeled actin monomers. This concept, called "bioprinting," has many potential applications in cell and tissue engineering.
Also in 2012, JoVE Bioengineering featured a protocol by Hsia et al. for isolating and purifying spider silk protein, spinning it into fibers, and assessing the fiber strength. This protocol for laboratory-scale production of spider silk, which is stronger than tensile steel, can potentially be extended to large-scale manufacturing.
In JoVE Clinical and Translational Medicine, we featured a number of articles related to stroke, one of which. In one article, M&ouml;bius-Winkler et al. demonstrated placement of the WATCHMAN left atrial appendage occlusion device from Boston Scientific. This device is designed to prevent strokes by trapping clots within the left atrial appendage of the heart before they exit. Our authors demonstrated how to insert the guide wire through the femoral artery into the heart, advance the device into the left atrium, and deploy the device in the left atrial appendage.
Another article focused on stroke, Hamel et al. described the use of a driving simulator to study how patients compensate for visual field defects following stroke. By analyzing the compensatory gaze behavior of patients as they navigated through virtual driving courses with varying degrees of complexity, our authors see great potential for the use of driving simulators in stroke rehabilitation.
In JoVE Immunology and Infection, we published an article by Keyel et al. describing the real-time kinetics of immune cell responses to bacterial toxins using live cell microscopy. Combined with high-speed 3D confocal microscopy, this technique can also visualize the cellular repair response.
Also in JoVE Immunology and Infection, we featured an article by Yap et al. describing methods for diagnosing helminth infections in children. Helminths are parasitic worms that can infect the intestinal tract of humans and animals, and may be distinguished based on their morphology. The same article demonstrated how to measure the impact of helminth infections on physical fitness in children, which is a measure of overall health.
In JoVE Neuroscience, we featured a method by Hoffmann et al., who used tiny headphones to alter the auditory feedback in songbirds, and analyzed the computational and neurophysiological basis of vocal learning in birds when they adjust their singing in response to altered acoustic signals.
Also in JoVE Neuroscience, we moved from birds to bees with a method that assesses associative and non-associative tactile learning in honeybees. This method, by Mujagi&#263; et al., allowed bees to scan different metal surfaces with their antennae, then conditioned them to expect sugar water when their antennae touched particular surfaces, and analyzed the corresponding changes in antennal movement.
In 2012, we launched a brand-new section: JoVE Applied Physics, which features articles on subjects ranging from plasma physics to material science. The Applied Physics section also contained JoVE's 2000th video article, filmed in a synchrotron radiation facility, where Borisenko et al. showed how to determine the electronic structure of complex materials using angle-resolved photoemission spectroscopy.
In the JoVE General section, we published an article by Artioli et al. demonstrating the use of a portable gas analyzer to measure oxygen consumption during complex exercise - such as judo. By analyzing oxygen consumption and collecting blood for measuring plasma lactate concentration, this method can determine the relative contributions of different energy systems to specific aspects of complex exercise.
Finally, JoVE followed Dolhi et al. to a permanently ice-covered saline lake in Antarctica. There, scientists drilled through the ice to study single-celled microorganisms called protists that live in the harsh conditions of Antarctica.
This Year in Review was just a brief glimpse of a few of over 600 video articles that JoVE offered in 2012. Browse the JoVE archives to see thousands of other videos, and stay tuned for what's coming up this year in JoVE: The Journal of Visualized Experiments.

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).

 
 
Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE ...

Methods Article

November 2016 - This Month in JoVE: Turtle tracking, acids in coffee, squid coloration and glucose tolerance in primates