Manufacturing of a Nafion-coated, Reduced Graphene Oxide/Polyaniline Chemiresistive Sensor to Monitor pH in Real-time During Microbial Fermentation

8.4K Views

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11:18 min

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January 7th, 2019

DOI :

10.3791/58422-v

January 7th, 2019

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Selvaraj Chinnathambi¹, Gert-Jan Euverink¹

¹Products and Processes for Biotechnology, Engineering and Technology Institute Groningen, Faculty of Science and Engineering, University of Groningen

Summary

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Keywords Nafion

Reduced Graphene Oxide

Polyaniline

Chemiresistive Sensor

PH Monitoring

Microbial Fermentation

Bioprocess Technology

Interdigitated Gold Electrode

Chapters in this video

0:04

Title

0:52

Preparation of Graphite Oxide

2:11

GO-deposited Electrode Preparation

3:07

Reduction of GO to Electrochemically Reduced Graphene Oxide

4:29

Polyaniline Functionalization of the ERGO Electrode

6:09

ERGO-PA Electrode Testing at Different pH (Pre-calibration Before Nafion Coating)

6:46

Preparation of the Nafion-coated ERGO-PA Electrode

7:22

Preparation of L. lactis Culture Medium

8:23

Testing of the ERGO-PA-NA pH Response in an L. lactis Fermentation Experiment

9:08

Results: Fabrication of ERGO-PA-NA Electrodes to Detect pH Changes During Microbial Fermentation

10:41

Conclusion

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