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The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions

DOI :

10.3791/55414-v

February 1st, 2018

February 1st, 2018

8,792 Views

1Life Science Department, University of Liège, 2Institute of Condensed Matter and Nanoscience, Catholic University of Louvain, 3Laboratory of Clinical Microbiology, Catholic University of Louvain, 4Biotechnology Department, CER Group

In this work, we report a new method to study protein-protein interactions using a conductimetric biosensor based on the hybrid β-lactamase technology. This method relies on release of protons upon hydrolysis of β-lactams.

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Conductimetric Biosensor Assay

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