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DOI :
10.3791/59156-v
•
7:33 min
March 19th, 2019
Chapters
0:04
Title
0:43
High-pressure Freezing
1:32
Freeze Substitution
2:39
Microwave-assisted Processing
4:10
Minimal Resin Embedding
4:43
Preparation for FIB-SEM (Focused Ion Beam Scanning Electron Microscope)
5:36
"
6:55
Conclusion
本文提出了一种结合高压冷冻和微波辅助样品处理两种样品处理技术的协议, 然后用聚焦离子束扫描电子显微镜 (FIB-SEM) 进行最小树脂包埋, 用于获取数据。这是用小鼠胫骨神经样本和线虫证明的。
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