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Predicting In Vivo Payloads Delivery using a Blood-brain Tumor-barrier in a Dish

DOI :

10.3791/59384-v

13:34 min

April 16th, 2019

April 16th, 2019

8,714 Views

1Research Programs Unit, Translational Cancer Medicine Research Program, University of Helsinki, 2Wihuri Research Institute, Biomedicum Helsinki, University of Helsinki, 3Laboratory Animal Center, Helsinki Institute of Life Science (HiLIFE), University of Helsinki

Drug targeting to central nervous system tumors is a major challenge. Here we describe a protocol to produce an in vitro mimic of the blood-brain tumor-barrier using murine and/or human cells and discuss their relevance for the predictability of central nervous system tumor targeting in vivo.

Tags

Involves Careful Handling And Positioning The Use Of Fluorescent Dyes And Microscopy Techniques Allows For Real time Visualization And Assessment Of The Compounds Diffusion And Targeting Capabilities This Method Provides Valuable Insights Into The Effectiveness Of Drug Delivery To The Brain Tumor Site

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