Using Computer-based Image Analysis to Improve Quantification of Lung Metastasis in the 4T1 Breast Cancer Model
6.1K Views
•
08:32 min
•
October 2nd, 2020
DOI :
October 2nd, 2020
•Margaret A. Nagai-Singer1, Alissa Hendricks-Wenger1,2,3, Rebecca M. Brock1,2, Holly A. Morrison1, Juselyn D. Tupik1, Sheryl Coutermarsh-Ott1, Irving C. Allen1,2,4
1Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, 2Graduate Program in Translational Biology, Medicine and Health, Virginia Polytechnic Institute and State University, 3Department of Biomedical Engineering and Mechanics, Virginia Polytechnic Institute and State University, 4Department of Basic Science Education, Virginia Tech Carilion School of Medicine, Virginia Polytechnic Institute and State University
Chapters in this video
0:04
Introduction
0:51
Processing Tissues
2:49
Staining Plates
4:01
Image Analysis
6:26
Results: Fiji-ImageJ Analysis is Accurate and Precise in Determining Metastatic Burden
8:04
Conclusion
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