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Ex Vivo Expansion of Hematopoietic Stem Cells from Human Umbilical Cord Blood-derived CD34+ Cells Using Valproic Acid

DOI :

10.3791/59532-v

April 11th, 2019

April 11th, 2019

9,526 Views

1Division of Hematology/Oncology, Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai

Here, we describe the ex vivo expansion of hematopoietic stem cells from CD34+ cells derived from umbilical cord blood treated with a combination of a cytokine cocktail and VPA. This method leads to a significant degree of ex vivo expansion of primitive HSCs for either clinical or laboratory applications.

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Ex Vivo Expansion

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